Product Description: diphthamide biosynthesis protein 3, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.3.1.14 (Diphthine--ammonia ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
| Function | GO:0008270 | zinc ion binding |
| Process | GO:0017183 | peptidyl-diphthamide biosynthetic process from peptidyl-histidine |
| Process | GO:0002098 | tRNA wobble uridine modification |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.01 | 0.99 |
| Trophozoite | not expressed | 0.01 | 0.99 |
| Schizont | not expressed | 0.01 | 0.99 |
| Gametocyte | not expressed | 0.04 | 0.98 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1217800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.169 | MFS: 0 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5N0
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.3.1.14 | diphthine-ammonia ligase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_103160
Most Similar Human Ortholog: Q96FX2
TM-align score: 0.71 | RMSD: 1.72
Seq Identity: 0.48 | Length: 67 / 80
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000154813 | diphthamide biosynthesis 3 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 35
Non-coding: 31 | Synonymous: 3 | Nonsynonymous: 1 | Stop Codon: 0
Protein Length: 80 | Molecular Weight (kDa): 9.374
UniProt ID(s): Q8I5N0
PDB ID(s): None
Isoelectric Point: 3.96
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR007872 | DPH-type metal-binding domain |
| InterPro | IPR036671 | DPH-type metal-binding domain superfamily |
| PFam | PF05207 | DPH-type metal-binding domain |
| Superfamily | SSF144217 | DPH-type metal-binding domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 28775286 | Elp3 and Dph3 of Schizosaccharomyces pombe mediate cellular stress responsesthrough tRNA(Lys)(UUU) modifications | Villahermosa D, Fleck O | 10.1038/s41598-017-07647-1 |
| 28555368 | A mutated dph3 gene causes sensitivity of Schizosaccharomyces pombe cells tocytotoxic agents | Villahermosa D, Knapp K, Fleck O | 10.1007/s00294-017-0711-x |
| 37662670 | Yeast gene KTI13 (alias DPH8) operates in the initiation step of diphthamidesynthesis on elongation factor 2 | Arend M, Utkur K, ..., Schaffrath R | 10.15698/mic2023.09.804 |