Product Description: thrombospondin-related apical membrane protein
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005794 | Golgi apparatus |
| Component | GO:0009986 | cell surface |
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0020009 | microneme |
| Component | GO:0005886 | plasma membrane |
| Component | GO:0020008 | rhoptry |
| Function | GO:0002020 | protease binding |
| Process | GO:0044409 | entry into host |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.48 | 0.81 |
| Trophozoite | possibly expressed | 0.09 | 0.94 |
| Schizont | expressed | 0.32 | 0.83 |
| Gametocyte | expressed | 0.24 | 0.86 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1218000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.224 | MFS: -2.612 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1433600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5M8
"Best" AlphaFill ligand hit: AR6 (adenosine-5-diphosphoribose, Local RMSD=7.37) with 4NA0 (Global RMSD=15.40)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 1 | 0 | 0 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 1 | 0 | 0 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
PlasmoDB Total SNPs: 114
Non-coding: 97 | Synonymous: 11 | Nonsynonymous: 5 | Stop Codon: 1
Protein Length: 352 | Molecular Weight (kDa): 41.261
UniProt ID(s): Q8I5M8
PDB ID(s): None
Isoelectric Point: 5.3
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000884 | Thrombospondin type-1 (TSP1) repeat |
| InterPro | IPR036383 | Thrombospondin type-1 (TSP1) repeat superfamily |
| PFam | PF00090 | Thrombospondin type-1 (TSP1) repeat |
| Superfamily | SSF82895 | Thrombospondin type-1 (TSP1) repeat superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12228305 | Independent translocation of two micronemal proteins in developing Plasmodiumfalciparum merozoites. | Healer J, Crawford S, ..., Cowman AF | 10.1128/IAI.70.10.5751-5758.2002 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 15003842 | PTRAMP; a conserved Plasmodium thrombospondin-related apical merozoite protein. | Thompson J, Cooke RE, ..., Waters AP | 10.1016/j.molbiopara.2003.12.003 |
| 16879884 | Plasmodium thrombospondin related apical merozoite protein (PTRAMP) is shed fromthe surface of merozoites by PfSUB2 upon invasion of erythrocytes. | Green JL, Hinds L, ..., Holder AA | 10.1016/j.molbiopara.2006.06.010 |
| 22467743 | Analysis of the conformation and function of the Plasmodium falciparum merozoiteproteins MTRAP and PTRAMP. | Uchime O, Herrera R, ..., Narum DL | 10.1128/EC.00039-12 |
| 23387921 | A thrombospondin structural repeat containing rhoptry protein from Plasmodiumfalciparum mediates erythrocyte invasion. | Siddiqui FA, Dhawan S, ..., Chitnis CE | 10.1111/cmi.12118 |