Product Description: arginine--tRNA ligase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.1.1.19 (Arginine--tRNA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0004814 | arginine-tRNA ligase activity |
| Process | GO:0006420 | arginyl-tRNA aminoacylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.28 | 0.86 |
| Trophozoite | expressed | 0.42 | 0.75 |
| Schizont | expressed | 0.15 | 0.90 |
| Gametocyte | expressed | 0.20 | 0.88 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1218600
Resistome Missense Mutations: K386Q
Resistome Compounds with Missense Mutations: 3-Hydroxypropanamidine 22
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -2.898 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1434200)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5M2
"Best" AlphaFill ligand hit: GGB (l-canavanine, Local RMSD=0.36) with 4Q2X (Global RMSD=3.18)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.1.1.19 | arginine-tRNA ligase | ADPAMPKClRNAiheminK2SO4ornithinecitrullinediphosphate8-azido-ATPhomoarginineRNA minihelix... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P54136 | Arginine--tRNA ligase, cytoplasmic | Homo sapiens | HOGENOM, OMA, OrthoDB, OrthoMCL BLAST | Aminoalkyl adenylate and aminoacyl sulfamate analogueCHEMBL434782 |
Ortholog Group (OrthoMCL): OG6_100350
Most Similar Human Ortholog: P54136
TM-align score: 0.92 | RMSD: 2.30
Seq Identity: 0.36 | Length: 575 / 590
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000113643 | arginyl-tRNA synthetase 1 |
| ENSG00000146282 | arginyl-tRNA synthetase 2, mitochondrial |
Protein Length: 590 | Molecular Weight (kDa): 68.436
UniProt ID(s): Q8I5M2
PDB ID(s): 5JLD
Isoelectric Point: 6.85
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001278 | Arginine-tRNA ligase |
| InterPro | IPR001412 | Aminoacyl-tRNA synthetase, class I, conserved site |
| InterPro | IPR005148 | Arginyl tRNA synthetase N-terminal domain |
| InterPro | IPR008909 | DALR anticodon binding |
| InterPro | IPR009080 | Aminoacyl-tRNA synthetase, class Ia, anticodon-binding |
| InterPro | IPR035684 | Arginyl-tRNA synthetase, catalytic core domain |
| InterPro | IPR036695 | Arginyl tRNA synthetase N-terminal domain superfamily |
| PFam | PF00750 | Arginyl-tRNA synthetase, catalytic core domain |
| PFam | PF03485 | Arginyl tRNA synthetase N-terminal domain |
| PFam | PF05746 | DALR anticodon binding |
| Superfamily | SSF47323 | Aminoacyl-tRNA synthetase, class Ia, anticodon-binding |
| Superfamily | SSF52374 | Arginyl tRNA synthetase N-terminal domain superfamily |
| Superfamily | SSF55190 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 29 | 13 | 38 |
| disruptive | 8 | 31 | 14 | 56 |
| missense | 8 | 31 | 14 | 48 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 61 | 23 | 36 |
| disruptive | 15 | 72 | 33 | 116 |
| missense | 13 | 61 | 29 | 82 |
PlasmoDB Total SNPs: 275
Non-coding: 230 | Synonymous: 38 | Nonsynonymous: 7 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 27502052 | Dimerization of Arginyl-tRNA Synthetase by Free Heme Drives Its Inactivation inPlasmodium falciparum. | Jain V, Yogavel M, Sharma A | 10.1016/j.str.2016.06.018 |