Product Description: phospholipid-transporting ATPase 2
SignalP Peptide: N/A
# Transmembrane Domains: 10
EC Numbers: 3.6.3.1 (Transferred entry: 7.6.2.1);7.6.2.1 (P-type phospholipid transporter)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0016021 | integral component of membrane |
| Component | GO:0005886 | plasma membrane |
| Function | GO:0016887 | ATP hydrolysis activity |
| Function | GO:0140326 | ATPase-coupled intramembrane lipid transporter activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0045332 | phospholipid translocation |
| Process | GO:0015914 | phospholipid transport |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.61 | 0.71 |
| Trophozoite | expressed | 1.03 | 0.47 |
| Schizont | expressed | 0.98 | 0.46 |
| Gametocyte | expressed | 0.51 | 0.71 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1219600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.119 | MFS: -3.033 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1434800)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1434800)
Modification: Tagged | RMgm-1405
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5L4
"Best" AlphaFill ligand hit: PCW ((z,z)-4-hydroxy-n,n,n-trimethyl-10-oxo-7-[(1-oxo-9-octadecenyl)oxy]-3,5,9-trioxa-4-phosphaheptacos-18-en-1-aminium-4-oxide, Local RMSD=0.04) with 7W48 (Global RMSD=2.88)
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100089
Most Similar Human Ortholog: H7C4E6
TM-align score: 0.95 | RMSD: 1.46
Seq Identity: 0.35 | Length: 237 / 1555
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000058063 | ATPase phospholipid transporting 11B (putative) |
| ENSG00000068650 | ATPase phospholipid transporting 11A |
| ENSG00000081923 | ATPase phospholipid transporting 8B1 |
| ENSG00000101974 | ATPase phospholipid transporting 11C |
| ENSG00000104043 | ATPase phospholipid transporting 8B4 (putative) |
| ENSG00000124406 | ATPase phospholipid transporting 8A1 |
| ENSG00000130270 | ATPase phospholipid transporting 8B3 |
| ENSG00000132932 | ATPase phospholipid transporting 8A2 |
| ENSG00000143515 | ATPase phospholipid transporting 8B2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 17 | 58 | 27 | 84 |
| disruptive | 20 | 51 | 46 | 101 |
| missense | 14 | 46 | 38 | 88 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 32 | 90 | 49 | 80 |
| disruptive | 35 | 137 | 59 | 190 |
| missense | 21 | 102 | 49 | 143 |
PlasmoDB Total SNPs: 144
Non-coding: 64 | Synonymous: 60 | Nonsynonymous: 20 | Stop Codon: 0
Protein Length: 1555 | Molecular Weight (kDa): 179.135
UniProt ID(s): Q27720, Q27728, Q8I5L4, Q9U421
PDB ID(s): None
Isoelectric Point: 7.07
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001757 | P-type ATPase |
| InterPro | IPR006539 | P-type ATPase, subfamily IV |
| InterPro | IPR008250 | P-type ATPase, A domain superfamily |
| InterPro | IPR018303 | P-type ATPase, phosphorylation site |
| InterPro | IPR023298 | P-type ATPase, transmembrane domain superfamily |
| InterPro | IPR023299 | P-type ATPase, cytoplasmic domain N |
| InterPro | IPR026871 | Phospholipid-transporting P-type ATPase |
| InterPro | IPR032630 | P-type ATPase, C-terminal |
| InterPro | IPR032631 | P-type ATPase, N-terminal |
| InterPro | IPR036412 | HAD-like superfamily |
| PFam | PF16209 | P-type ATPase, N-terminal |
| PFam | PF16212 | P-type ATPase, C-terminal |
| Superfamily | SSF56784 | HAD-like superfamily |
| Superfamily | SSF81653 | P-type ATPase, A domain superfamily |
| Superfamily | SSF81660 | P-type ATPase, cytoplasmic domain N |
| Superfamily | SSF81665 | P-type ATPase, transmembrane domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 11152613 | Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes. | Krogh A, Larsson B, von Heijne G, Sonnhammer EL | 10.1006/jmbi.2000.4315 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 29326268 | Mapping the malaria parasite druggable genome by using in vitro evolution andchemogenomics. | Cowell AN, Istvan ES, ..., Winzeler EA | 10.1126/science.aan4472 |
| 33372863 | ATP2, The essential P4-ATPase of malaria parasites, catalyzes lipid-stimulatedATP hydrolysis in complex with a Cdc50 beta-subunit. | Lamy A, Macarini-Bruzaferro E, ..., Vazquez-Ibar JL | https://pubmed.ncbi.nlm.nih.gov/33372863/ |
| 35862778 | CDC50 Orthologues in Plasmodium falciparum Have Distinct Roles in MerozoiteEgress and Trophozoite Maturation. | Patel A, Nofal SD, Blackman MJ, Baker DA | 10.1128/mbio.01635-22 |
| 35877905 | In Silico Assessment of the Lipid Fingerprint Signature of ATP2, the EssentialP4-ATPase of Malaria Parasites | Lopez-Martin M, Renault P, ..., Peralvarez-Marin A | 10.3390/membranes12070702 |