Product Description: pre-mRNA-processing factor 17, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005681 | spliceosomal complex |
| Process | GO:0000375 | RNA splicing, via transesterification reactions |
| Process | GO:0000350 | generation of catalytic spliceosome for second transesterification step |
| Process | GO:0000389 | mRNA 3'-splice site recognition |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.27 | 0.87 |
| Trophozoite | expressed | 0.44 | 0.74 |
| Schizont | expressed | 0.11 | 0.93 |
| Gametocyte | expressed | 0.28 | 0.83 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1220100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.225 | MFS: -2.701 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1435300)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5L1
"Best" AlphaFill ligand hit: ALQ (2-methyl-propionic acid, Local RMSD=0.28) with 4GM3 (Global RMSD=1.87)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102256
Most Similar Human Ortholog: O60508
TM-align score: 0.59 | RMSD: 2.97
Seq Identity: 0.40 | Length: 367 / 618
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000168438 | cell division cycle 40 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 38 | 20 | 26 |
| disruptive | 7 | 56 | 42 | 82 |
| missense | 7 | 53 | 41 | 70 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 24 | 58 | 14 | 32 |
| disruptive | 17 | 158 | 75 | 133 |
| missense | 12 | 135 | 72 | 94 |
PlasmoDB Total SNPs: 114
Non-coding: 72 | Synonymous: 32 | Nonsynonymous: 10 | Stop Codon: 0
Protein Length: 618 | Molecular Weight (kDa): 72.536
UniProt ID(s): Q8I5L1
PDB ID(s): None
Isoelectric Point: 7.03
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001680 | WD40 repeat |
| InterPro | IPR020472 | G-protein beta WD-40 repeat |
| InterPro | IPR032847 | Pre-mRNA-processing factor 17 |
| InterPro | IPR036322 | WD40-repeat-containing domain superfamily |
| PFam | PF00400 | WD40 repeat |
| Superfamily | SSF50978 | WD40-repeat-containing domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 28076346 | Cryo-EM structure of a human spliceosome activated for step 2 of splicing | Bertram K, Agafonov DE, ..., Luhrmann R | 10.1038/nature21079 |
| 21386897 | Systematic two-hybrid and comparative proteomic analyses reveal novel yeastpre-mRNA splicing factors connected to Prp19 | Ren L, McLean JR, ..., Gould KL | 10.1371/journal.pone.0016719 |
| 28919079 | Structure of an Intron Lariat Spliceosome from Saccharomyces cerevisiae | Wan R, Yan C, ..., Shi Y | 10.1016/j.cell.2017.08.029 |
| 27980089 | Structure of a yeast step II catalytically activated spliceosome | Yan C, Wan R, ..., Shi Y | 10.1126/science.aak9979 |
| 25524563 | The spliceosomal PRP19 complex of trypanosomes | Ambrosio DL, Badjatia N, Gunzl A | 10.1111/mmi.12910 |
| 17171376 | A role for the yeast cell cycle/splicing factor Cdc40 in the G1/S transition | Kaplan Y, Kupiec M | 10.1007/s00294-006-0113-y |
| 15133121 | The Saccharomyces cerevisiae gene CDC40/PRP17 controls cell cycle progressionthrough splicing of the ANC1 gene | Dahan O, Kupiec M | 10.1093/nar/gkh574 |
| 15452114 | Genome-wide analysis of pre-mRNA splicing: intron features govern the requirementfor the second-step factor, Prp17 in Saccharomyces cerevisiae andSchizosaccharomyces pombe | Sapra AK, Arava Y, Khandelia P, Vijayraghavan U | 10.1074/jbc.M408815200 |
| 12871902 | Genetic interactions with CLF1 identify additional pre-mRNA splicing factors anda link between activators of yeast vesicular transport and splicing | Vincent K, Wang Q, ..., Rymond BC | 10.1093/genetics/164.3.895 |
| 20419786 | PRP-17 and the pre-mRNA splicing pathway are preferentially required for theproliferation versus meiotic development decision and germline sex determinationin Caenorhabditis elegans | Kerins JA, Hanazawa M, Dorsett M, Schedl T | 10.1002/dvdy.22274 |