PF3D7_1220400 (DRN1)

Genome location: Pf3D7_12_v3:816,430..819,402(+)

Genome classification: Core

Function and Localization

Product Description: debranching enzyme-associated ribonuclease, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0071014	post-mRNA release spliceosomal complex
Process	GO:0000398	mRNA splicing, via spliceosome

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.30	0.85
Trophozoite	expressed	0.38	0.77
Schizont	expressed	0.35	0.78
Gametocyte	expressed	0.73	0.60

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1220400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.779 | MFS: -2.514 | #Insertions: 1

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1435600)

Relative Growth Rate: 0.05 ± 0.04
Confidence: 8.02

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I5K9

"Best" AlphaFill ligand hit: BMF (bromosporine, Local RMSD=0.68) with 5U9N (Global RMSD=18.76)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103311

Most Similar Human Ortholog: Q2TBE0

TM-align score: 0.51 | RMSD: 4.71

Seq Identity: 0.23 | Length: 315 / 519

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000152404	CWF19 like cell cycle control factor 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	16	7	24
disruptive	15	73	55	90
missense	10	61	52	79

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	31	17	35
disruptive	42	158	59	149
missense	24	132	48	99

PlasmoDB Total SNPs: 124

Non-coding: 79 | Synonymous: 32 | Nonsynonymous: 13 | Stop Codon: 0

Protein Information

Protein Length: 519 | Molecular Weight (kDa): 62.716

UniProt ID(s): Q8I5K9

PDB ID(s): None

Isoelectric Point: 9.47

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006767	Cwf19-like protein, C-terminal domain-2
InterPro	IPR006768	Cwf19-like, C-terminal domain-1
InterPro	IPR040194	Cwf19-like protein
PFam	PF04676	Cwf19-like protein, C-terminal domain-2
PFam	PF04677	Cwf19-like, C-terminal domain-1
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
25009170	Genetic basis for Saccharomyces cerevisiae biofilm in liquid medium	Scherz K, Andersen, ..., Regenberg B	10.1534/g3.114.010892