Product Description: ribosome biogenesis protein TSR3, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.5.1.- (Transferring alkyl or aryl groups, other than methyl groups.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Process | GO:0030490 | maturation of SSU-rRNA |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.15 | 0.93 |
| Trophozoite | not expressed | 0.29 | 0.82 |
| Schizont | not expressed | 0.05 | 0.97 |
| Gametocyte | not expressed | 0.17 | 0.90 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1220500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.162 | MFS: -2.839 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5K8
"Best" AlphaFill ligand hit: EEM (se-adenosylselenomethionine, Local RMSD=0.80) with 5APG (Global RMSD=2.50)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.5.1.- | Ex: N1-aminopropylagmatine synthase | APHBPYS04070YS05035taxodoneJDS05119JDS05120celastroltaxodionearenaroneMMV019313bacitracinclodronate... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101679
Most Similar Human Ortholog: Q9UJK0
TM-align score: 0.55 | RMSD: 3.10
Seq Identity: 0.29 | Length: 193 / 318
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000007520 | TSR3 ribosome maturation factor |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 12 | 10 | 19 |
| disruptive | 6 | 26 | 16 | 54 |
| missense | 5 | 23 | 14 | 49 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 33 | 13 | 18 |
| disruptive | 13 | 67 | 42 | 91 |
| missense | 6 | 58 | 37 | 69 |
PlasmoDB Total SNPs: 32
Non-coding: 19 | Synonymous: 12 | Nonsynonymous: 1 | Stop Codon: 0
Protein Length: 318 | Molecular Weight (kDa): 37.942
UniProt ID(s): Q8I5K8
PDB ID(s): None
Isoelectric Point: 8.32
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR007177 | 16S/18S rRNA aminocarboxypropyltransferase Tsr3, C-terminal |
| InterPro | IPR007209 | RNase L inhibitor RLI, possible metal-binding domain |
| InterPro | IPR022968 | 16S/18S rRNA aminocarboxypropyltransferase Tsr3 |
| PFam | PF04034 | 16S/18S rRNA aminocarboxypropyltransferase Tsr3, C-terminal |
| PFam | PF04068 | RNase L inhibitor RLI, possible metal-binding domain |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 25566315 | Analysis of two domains with novel RNA-processing activities throws light on the complex evolution of ribosomal RNA biogenesis | Burroughs AM, Aravind L | 10.3389/fgene.2014.00424 |
| 27084949 | Ribosome biogenesis factor Tsr3 is the aminocarboxypropyl transferase responsiblefor 18S rRNA hypermodification in yeast and humans | Meyer B, Wurm JP, ..., Entian KD | 10.1093/nar/gkw244 |