Product Description: histone-lysine N-methyltransferase, H3 lysine-4 specific
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.1.1.43 (Transferred entry: 2.1.1.354)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0034399 | nuclear periphery |
| Component | GO:0005634 | nucleus |
| Function | GO:0042800 | histone methyltransferase activity (H3-K4 specific) |
| Process | GO:0020033 | antigenic variation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.11 | 0.95 |
| Trophozoite | expressed | 0.69 | 0.62 |
| Schizont | expressed | 1.06 | 0.51 |
| Gametocyte | expressed | 0.52 | 0.71 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1221000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.865 | #Insertions: 7
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1436200)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1436200)
Modification: Disrupted | RMgm-4630
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5K3
"Best" AlphaFill ligand hit: ABH (2(s)-amino-6-boronohexanoic acid, Local RMSD=0.11) with 3MMR (Global RMSD=37.93)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.1.1.43 | histone-lysine N-methyltransferase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 24 | 86 | 46 | 121 |
| disruptive | 129 | 257 | 134 | 333 |
| missense | 66 | 189 | 107 | 258 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 40 | 148 | 73 | 143 |
| disruptive | 183 | 484 | 212 | 445 |
| missense | 93 | 365 | 163 | 308 |
PlasmoDB Total SNPs: 600
Non-coding: 163 | Synonymous: 213 | Nonsynonymous: 224 | Stop Codon: 0
Protein Length: 2329 | Molecular Weight (kDa): 271.07
UniProt ID(s): Q8I5K3
PDB ID(s): None
Isoelectric Point: 6
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001965 | Zinc finger, PHD-type |
| InterPro | IPR011011 | Zinc finger, FYVE/PHD-type |
| InterPro | IPR019786 | Zinc finger, PHD-type, conserved site |
| PFam | N/A | N/A |
| Superfamily | SSF57903 | Zinc finger, FYVE/PHD-type |
| Superfamily | SSF82199 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 19765590 | Potential epigenetic regulatory proteins localise to distinct nuclearsub-compartments in Plasmodium falciparum. | Volz J, Carvalho TG, ..., Cowman AF | 10.1016/j.ijpara.2009.09.002 |
| 22264509 | PfSET10, a Plasmodium falciparum methyltransferase, maintains the active var genein a poised state during parasite division. | Volz JC, Bartfai R, ..., Cowman AF | 10.1016/j.chom.2011.11.011 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 33536326 | The Role of the Histone Methyltransferase Pf SET10 in Antigenic Variation by Malaria Parasites: a Cautionary Tale | Ngwa CJ, Gross MR, ..., Deitsch KW | 10.1128/mSphere.01217-20 |