PF3D7_1221600

Genome location: Pf3D7_12_v3:863,593..865,537(+)

Genome classification: Core

Function and Localization

Product Description: protein TSSC1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Process	GO:0016567	protein ubiquitination

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.03	0.98
Trophozoite	not expressed	0.32	0.80
Schizont	not expressed	0.10	0.93
Gametocyte	not expressed	0.19	0.89

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1221600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.169 | MFS: -2.695 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1436800)

Relative Growth Rate: 0.10 ± nan
Confidence: 1.00

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I5J7

"Best" AlphaFill ligand hit: SIW (keggin (sta), Local RMSD=0.14) with 6Y7O (Global RMSD=2.61)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103789

Most Similar Human Ortholog: Q53HC9

TM-align score: 0.83 | RMSD: 2.64

Seq Identity: 0.26 | Length: 350 / 458

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000032389	EARP complex and GARP complex interacting protein 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	14	8	21
disruptive	35	106	42	97
missense	22	72	26	71

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	28	11	31
disruptive	79	163	76	171
missense	42	110	56	91

PlasmoDB Total SNPs: 89

Non-coding: 28 | Synonymous: 25 | Nonsynonymous: 36 | Stop Codon: 0

Protein Information

Protein Length: 458 | Molecular Weight (kDa): 54.019

UniProt ID(s): Q8I5J7

PDB ID(s): None

Isoelectric Point: 6.27

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001680	WD40 repeat
InterPro	IPR019775	WD40 repeat, conserved site
InterPro	IPR036322	WD40-repeat-containing domain superfamily
InterPro	IPR040323	EARP and GARP complex-interacting protein 1
PFam	PF00400	WD40 repeat
Superfamily	SSF50978	WD40-repeat-containing domain superfamily