PF3D7_1221700

Genome location: Pf3D7_12_v3:865,726..868,762(+)

Genome classification: Core

Function and Localization

Product Description: FbpA domain protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 2

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:1990112	RQC complex
Component	GO:0020011	apicoplast
Function	GO:0043023	ribosomal large subunit binding
Function	GO:0000049	tRNA binding
Process	GO:0072344	rescue of stalled ribosome
Process	GO:1990116	ribosome-associated ubiquitin-dependent protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.09	0.95
Trophozoite	not expressed	0.48	0.72
Schizont	expressed	0.11	0.93
Gametocyte	not expressed	0.18	0.90

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1221700

Resistome Missense Mutations: M57R

Resistome Compounds with Missense Mutations: DSM1

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.159 | MFS: -3.294 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1436900)

Relative Growth Rate: 0.18 ± 0.08
Confidence: 6.48

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1436900)

Modification: Disrupted | RMgm-3927

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I5J6

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.41) with 1Y4S (Global RMSD=13.93)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_146176

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	7	36	33	45
disruptive	20	81	44	147
missense	12	52	37	113

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	9	79	37	53
disruptive	51	174	98	193
missense	25	116	66	128

PlasmoDB Total SNPs: 124

Non-coding: 36 | Synonymous: 61 | Nonsynonymous: 26 | Stop Codon: 1

Protein Information

Protein Length: 913 | Molecular Weight (kDa): 109.146

UniProt ID(s): Q8I5J6

PDB ID(s): None

Isoelectric Point: 9.27

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR008532	NFACT, RNA-binding domain
PFam	PF05670	NFACT, RNA-binding domain
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
12368868	Sequence of Plasmodium falciparum chromosomes 2, 10, 11 and 14.	Gardner MJ, Shallom SJ, ..., Fraser CM	10.1038/nature01094
30212465	Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites.	Boucher MJ, Ghosh S, ..., Yeh E	10.1371/journal.pbio.2005895