Product Description: AP2 domain transcription factor
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0043565 | sequence-specific DNA binding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.15 | 0.93 |
| Trophozoite | expressed | 1.00 | 0.49 |
| Schizont | expressed | 0.61 | 0.67 |
| Gametocyte | expressed | 0.97 | 0.52 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1222400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 3 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.997 | MFS: -2.553 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5J1
"Best" AlphaFill ligand hit: UDP (uridine-5'-diphosphate, Local RMSD=1.17) with 2VL8 (Global RMSD=33.63)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 27 | 98 | 61 | 112 |
| disruptive | 139 | 458 | 228 | 580 |
| missense | 84 | 323 | 188 | 444 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 46 | 191 | 93 | 169 |
| disruptive | 268 | 1000 | 443 | 916 |
| missense | 150 | 704 | 317 | 507 |
PlasmoDB Total SNPs: 478
Non-coding: 100 | Synonymous: 215 | Nonsynonymous: 160 | Stop Codon: 3
Protein Length: 2558 | Molecular Weight (kDa): 301.911
UniProt ID(s): Q8I5J1
PDB ID(s): None
Isoelectric Point: 7.3
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001471 | AP2/ERF domain |
| InterPro | IPR016181 | Acyl-CoA N-acyltransferase |
| PFam | PF00847 | AP2/ERF domain |
| Superfamily | SSF55729 | Acyl-CoA N-acyltransferase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 21060817 | Identification and genome-wide prediction of DNA binding specificities for theApiAP2 family of regulators from the malaria parasite. | Campbell TL, De Silva EK, ..., Llinas M | 10.1371/journal.ppat.1001165 |
| 34492224 | Single-genome sequencing reveals within-host evolution of human malariaparasites | Dia A, Jett C, ..., Cheeseman IH | 10.1016/j.chom.2021.08.009 |
| 37898053 | Cell cycle-regulated ApiAP2s and parasite development: the Toxoplasma paradigm | Zarringhalam K, Ye S, ..., Gubbels MJ | 10.1016/j.mib.2023.102383 |
| 35087229 | The transcriptional regulator HDP1 controls expansion of the inner membranecomplex during early sexual differentiation of malaria parasites | Campelo Morillo RA, Tong X, ..., Kafsack BFC | 10.1038/s41564-021-01045-0 |
| 35871979 | A transcription factor helps Plasmodium falciparum gametocytogenesis take shape | McLean KJ, Niles JC | 10.1016/j.pt.2022.07.004 |
| 38311493 | Sex in a dish: high-efficiency Toxoplasma pre-sexual development | Hryckowian ND, Knoll LJ | 10.1016/j.pt.2024.01.009 |
| 36292619 | Epigenetic and Epitranscriptomic Gene Regulation in Plasmodium falciparum and HowWe Can Use It against Malaria | Serrano-Duran R, Lopez-Farfan D, Gomez-Diaz E | 10.3390/genes13101734 |
| 37350586 | An apicomplexan bromodomain protein, TgBDP1, associates with diverse epigeneticfactors to regulate essential transcriptional processes in Toxoplasma gondii | Fleck K, McNutt S, Chu F, Jeffers V | 10.1128/mbio.03573-22 |
| 34880324 | Essential role of a Plasmodium berghei heat shock protein (PBANKA_0938300) ingametocyte development | Kashif M, Quadiri A, Singh AP | 10.1038/s41598-021-03059-4 |
| 36847501 | Single-Cell Transcriptomics To Define Plasmodium falciparum Stage Transition inthe Mosquito Midgut | Mohammed M, Dziedziech A, ..., Ankarklev J | 10.1128/spectrum.03671-22 |
| 38370614 | Structural characterization of the ACDC domain from ApiAP2 proteins of themalaria parasite | Le Berre M, Tubiana T, ..., Nessler S | 10.1101/2024.02.09.579679 |
| 35083215 | PfAP2-EXP2, an Essential Transcription Factor for the IntraerythrocyticDevelopment of Plasmodium falciparum | Shang X, Wang C, ..., Jiang M | 10.3389/fcell.2021.782293 |
| 34874774 | The Extracellular Milieu of Toxoplasma's Lytic Cycle Drives Lab Adaptation,Primarily by Transcriptional Reprogramming | Primo VA Jr, Rezvani Y, ..., Gubbels MJ | 10.1128/mSystems.01196-21 |
| 37293082 | PfAP2-MRP DNA-binding protein is a master regulator of parasite pathogenesisduring malaria parasite blood stages | Subudhi AK, Green JL, ..., Pain A | 10.1101/2023.05.23.541898 |
| 36223427 | Inhibitors of ApiAP2 protein DNA binding exhibit multistage activity againstPlasmodium parasites | Russell TJ, De Silva EK, ..., Llinas M | 10.1371/journal.ppat.1010887 |
| 35288749 | Genome-wide landscape of ApiAP2 transcription factors reveals aheterochromatin-associated regulatory network during Plasmodium falciparumblood-stage development | Shang X, Wang C, ..., Zhang Q | 10.1093/nar/gkac176 |
| 34400833 | A heat-shock response regulated by the PfAP2-HS transcription factor protectshuman malaria parasites from febrile temperatures | Tinto-Font E, Michel-Todo L, ..., Cortes A | 10.1038/s41564-021-00940-w |