Product Description: diphthine--ammonia ligase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.3.1.14 (Diphthine--ammonia ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0017178 | diphthine-ammonia ligase activity |
| Process | GO:0017183 | peptidyl-diphthamide biosynthetic process from peptidyl-histidine |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.13 | 0.93 |
| Trophozoite | expressed | 0.12 | 0.92 |
| Schizont | expressed | 0.13 | 0.91 |
| Gametocyte | expressed | 0.22 | 0.87 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1222500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.149 | MFS: -3.171 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5J0
"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=3.78) with 3RK1 (Global RMSD=2.83)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.3.1.14 | diphthine-ammonia ligase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101395
Most Similar Human Ortholog: Q7L8W6
TM-align score: 0.98 | RMSD: 0.83
Seq Identity: 0.45 | Length: 246 / 247
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000134146 | diphthamine biosynthesis 6 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 0 | 1 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 1 | 0 |
| disruptive | 1 | 0 | 0 | 1 |
| missense | 0 | 0 | 0 | 0 |
PlasmoDB Total SNPs: 337
Non-coding: 318 | Synonymous: 17 | Nonsynonymous: 2 | Stop Codon: 0
Protein Length: 247 | Molecular Weight (kDa): 28.481
UniProt ID(s): Q8I5J0
PDB ID(s): None
Isoelectric Point: 6.23
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002761 | Diphthamide synthase domain |
| InterPro | IPR030662 | Diphthine--ammonia ligase/Uncharacterised protein MJ0570 |
| PFam | PF01902 | Diphthamide synthase domain |
| Superfamily | SSF52402 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23468660 | The amidation step of diphthamide biosynthesis in yeast requires DPH6, a geneidentified through mining the DPH1-DPH5 interaction network | Uthman S, Bar C, ..., Schaffrath R | 10.1371/journal.pgen.1003334 |