Product Description: AP2 domain transcription factor AP2-G
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Function | GO:0001216 | DNA-binding transcription activator activity |
| Function | GO:0005515 | protein binding |
| Function | GO:0043565 | sequence-specific DNA binding |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.26 | 0.89 |
| Trophozoite | possibly expressed | 0.13 | 0.93 |
| Schizont | expressed | 0.35 | 0.85 |
| Gametocyte | not expressed | 0.19 | 0.90 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1222600
Resistome Missense Mutations: S2162R, K2207N
Resistome Compounds with Missense Mutations: GNF-Pf-3600, TCMDC-124553/MMV019869
Resistome # Samples with Disruptive Mutations: 35 (2 missense, 2 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.72 | #Insertions: 3
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1437500)
Modification: Tagged | RMgm-5029
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5I9
"Best" AlphaFill ligand hit: HEM (heme, Local RMSD=5.31) with 4NKX (Global RMSD=30.75)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 101 | 53 | 127 |
| disruptive | 96 | 364 | 190 | 461 |
| missense | 72 | 268 | 148 | 349 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 43 | 210 | 94 | 182 |
| disruptive | 233 | 757 | 383 | 891 |
| missense | 152 | 544 | 292 | 492 |
PlasmoDB Total SNPs: 326
Non-coding: 47 | Synonymous: 156 | Nonsynonymous: 122 | Stop Codon: 1
Protein Length: 2432 | Molecular Weight (kDa): 284.064
UniProt ID(s): Q8I5I9
PDB ID(s): None
Isoelectric Point: 8.65
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001471 | AP2/ERF domain |
| PFam | PF00847 | AP2/ERF domain |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 21060817 | Identification and genome-wide prediction of DNA binding specificities for theApiAP2 family of regulators from the malaria parasite. | Campbell TL, De Silva EK, ..., Llinas M | 10.1371/journal.ppat.1001165 |
| 24572369 | A transcriptional switch underlies commitment to sexual development in malariaparasites. | Kafsack BF, Rovira-Graells N, ..., Llinas M | 10.1038/nature12920 |
| 29326268 | Mapping the malaria parasite druggable genome by using in vitro evolution andchemogenomics. | Cowell AN, Istvan ES, ..., Winzeler EA | 10.1126/science.aan4472 |
| 32198457 | Dissecting the role of PfAP2-G in malaria gametocytogenesis. | Josling GA, Russell TJ, ..., Llinas M | 10.1038/s41467-020-15026-0 |
| 33268801 | The transcriptome of circulating sexually committed Plasmodium falciparum ringstage parasites forecasts malaria transmission potential. | Prajapati SK, Ayanful-Torgby R, ..., Williamson KC | 10.1038/s41467-020-19988-z |
| 35947628 | Identification of a novel AP2 transcription factor in zygotes with an essentialrole in Plasmodium ookinete development | Nishi T, Kaneko I, Iwanaga S, Yuda M | 10.1371/journal.ppat.1010510 |
| 34119684 | Mechanisms of triggering malaria gametocytogenesis by AP2-G | Yuda M, Kaneko I, ..., Nishi T | 10.1016/j.parint.2021.102403 |
| 34261498 | MALBoost: a web-based application for gene regulatory network analysis inPlasmodium falciparum | van Wyk R, van Biljon R, Birkholtz LM | 10.1186/s12936-021-03848-2 |
| 31086187 | Plasmodium falciparum sexual differentiation in malaria patients is associatedwith host factors and GDV1-dependent genes | Usui M, Prajapati SK, ..., Williamson KC | 10.1038/s41467-019-10172-6 |
| 33903595 | Higher gametocyte production and mosquito infectivity in chronic compared toincident Plasmodium falciparum infections | Barry A, Bradley J, ..., Bousema T | 10.1038/s41467-021-22573-7 |
| 34011691 | A 39-Amino-Acid C-Terminal Truncation of GDV1 Disrupts Sexual Commitment inPlasmodium falciparum | Tiburcio M, Hitz E, ..., Treeck M | 10.1128/mSphere.01093-20 |
| 36409095 | Plasmodium falciparum Sexual Commitment Rate Variation among Clinical Isolatesand Diverse Laboratory-Adapted Lines | Stewart LB, Freville A, ..., Conway DJ | 10.1128/spectrum.02234-22 |
| 31734953 | A patatin-like phospholipase functions during gametocyte induction in the malariaparasite Plasmodium falciparum | Flammersfeld A, Panyot A, ..., Pradel G | 10.1111/cmi.13146 |
| 33053503 | Metabolic regulation of sexual commitment in Plasmodium falciparum | Neveu G, Beri D, Kafsack BF | 10.1016/j.mib.2020.09.004 |
| 37681121 | Sequence-dependent heterochromatin formation in the human malaria parasitePlasmodium falciparum | Mori T, Nakashima M | 10.1016/j.heliyon.2023.e19164 |
| 36711670 | Global gene expression of human malaria parasite liver stages throughoutintrahepatocytic development | Zanghi G, Patel H, ..., Kappe SHI | 10.1101/2023.01.05.522945 |
| 34232098 | Single-Cell RNA Sequencing Reveals Cellular Heterogeneity and Stage Transitionunder Temperature Stress in Synchronized Plasmodium falciparum Cells | Rawat M, Srivastava A, ..., Karmodiya K | 10.1128/Spectrum.00008-21 |
| 37277533 | Sexual differentiation in human malaria parasites is regulated by competitionbetween phospholipid metabolism and histone methylation | Harris CT, Tong X, ..., Kafsack BFC | 10.1038/s41564-023-01396-w |
| 36916164 | Plasmodium falciparum adapts its investment into replication versus transmissionaccording to the host environment | Abdi AI, Achcar F, ..., Marti M | 10.7554/eLife.85140 |
| 33425787 | From Genes to Transcripts, a Tightly Regulated Journey in Plasmodium | Hollin T, Le Roch KG | 10.3389/fcimb.2020.618454 |
| 32487761 | Rrp6 Regulates Heterochromatic Gene Silencing via ncRNA RUF6 Decay in MalariaParasites | Fan Y, Shen S, ..., Zhang Q | 10.1128/mBio.01110-20 |
| 33794978 | H3K36 methylation reprograms gene expression to drive early gametocytedevelopment in Plasmodium falciparum | Connacher J, Josling GA, ..., Birkholtz LM | 10.1186/s13072-021-00393-9 |
| 35950755 | Highly Variable Expression of Merozoite Surface Protein MSPDBL2 in DiversePlasmodium falciparum Clinical Isolates and Transcriptome Scans for CorrelatingGenes | Hocking SE, Stewart LB, ..., Conway DJ | 10.1128/mbio.01948-22 |
| 36634679 | Regulators of male and female sexual development are critical for thetransmission of a malaria parasite | Russell AJC, Sanderson T, ..., Billker O | 10.1016/j.chom.2022.12.011 |