Product Description: glideosome-associated protein 45
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0019898 | extrinsic component of membrane |
| Component | GO:0033644 | host cell membrane |
| Component | GO:0070258 | inner membrane pellicle complex |
| Function | GO:0005515 | protein binding |
| Process | GO:0044409 | entry into host |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.31 | 0.88 |
| Trophozoite | expressed | 0.27 | 0.83 |
| Schizont | expressed | 1.96 | 0.23 |
| Gametocyte | expressed | 0.68 | 0.63 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1222700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 1 | MFS: -1.808 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1437600)
Modification: Mutated | RMgm-776
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5I8
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 57
Non-coding: 51 | Synonymous: 6 | Nonsynonymous: 0 | Stop Codon: 0
Protein Length: 204 | Molecular Weight (kDa): 23.632
UniProt ID(s): Q8I5I8
PDB ID(s): None
Isoelectric Point: 4.18
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | N/A | N/A |
| PFam | N/A | N/A |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 16321976 | A conserved molecular motor drives cell invasion and gliding motility acrossmalaria life cycle stages and other apicomplexan parasites. | Baum J, Richard D, ..., Cowman AF | 10.1074/jbc.M509807200 |
| 19561073 | A novel family of Apicomplexan glideosome-associated proteins with an innermembrane-anchoring role. | Bullen HE, Tonkin CJ, ..., Gilson PR | 10.1074/jbc.M109.036772 |
| 19576251 | Effects of calcium signaling on Plasmodium falciparum erythrocyte invasion andpost-translational modification of gliding-associated protein 45 (PfGAP45). | Jones ML, Cottingham C, Rayner JC | 10.1016/j.molbiopara.2009.06.007 |
| 20951968 | Functional dissection of the apicomplexan glideosome molecular architecture. | Frenal K, Polonais V, ..., Soldati-Favre D | 10.1016/j.chom.2010.09.002 |
| 22479457 | Subcellular location, phosphorylation and assembly into the motor complex ofGAP45 during Plasmodium falciparum schizont development. | Ridzuan MA, Moon RW, ..., Green JL | 10.1371/journal.pone.0033845 |
| 25802338 | The Plasmodium Class XIV Myosin, MyoB, Has a Distinct Subcellular Location inInvasive and Motile Stages of the Malaria Parasite and an Unusual Light Chain. | Yusuf NA, Green JL, ..., Holder AA | 10.1074/jbc.M115.637694 |
| 29970464 | The Actinomyosin Motor Drives Malaria Parasite Red Blood Cell Invasion but Not Egress | Perrin AJ, Collins CR, ..., Blackman MJ | 10.1128/mBio.00905-18 |
| 24632839 | The toxoplasma Acto-MyoA motor complex is important but not essential for glidingmotility and host cell invasion | Egarter S, Andenmatten N, ..., Meissner M | 10.1371/journal.pone.0091819 |
| 21610105 | Unusual N-glycan structures required for trafficking Toxoplasma gondii GAP50 tothe inner membrane complex regulate host cell entry through parasite motility | Fauquenoy S, Hovasse A, ..., Tomavo S | 10.1074/mcp.M111.008953 |
| 37821727 | Phosphoproteomic Analysis Reveals the Predominating Cellular Processes and theInvolved Key Phosphoproteins Essential for the Proliferation of Toxoplasmagondii | Chen XZ, Bai RX, ..., He C | 10.1007/s11686-023-00720-y |
| 18768477 | The motor complex of Plasmodium falciparum: phosphorylation by acalcium-dependent protein kinase | Green JL, Rees-Channer RR, ..., Holder AA | 10.1074/jbc.M803129200 |
| 35775739 | Inner membrane complex proteomics reveals a palmitoylation regulation criticalfor intraerythrocytic development of malaria parasite | Qian P, Wang X, ..., Yuan J | 10.7554/eLife.77447 |
| 34695132 | Inhibition of protein N-myristoylation blocks Plasmodium falciparumintraerythrocytic development, egress and invasion | Schlott AC, Knuepfer E, ..., Holder AA | 10.1371/journal.pbio.3001408 |
| 30130043 | [Research Advances on Gliding-associated Proteins of Toxoplasma gondii] | Li RH, Yin GR | https://pubmed.ncbi.nlm.nih.gov/30130043/ |
| 27444499 | Characterization of a Toxoplasma gondii calcium calmodulin-dependent proteinkinase homolog | Kato K, Sugi T, ..., Akashi H | 10.1186/s13071-016-1676-1 |
| 25393004 | Plasticity between MyoC- and MyoA-glideosomes: an example of functionalcompensation in Toxoplasma gondii invasion | Frenal K, Marq JB, ..., Soldati-Favre D | 10.1371/journal.ppat.1004504 |
| 21980283 | Quantitative in vivo analyses reveal calcium-dependent phosphorylation sites andidentifies a novel component of the Toxoplasma invasion motor complex | Nebl T, Prieto JH, ..., Tonkin CJ | 10.1371/journal.ppat.1002222 |
| 34395314 | Calcium in the Backstage of Malaria Parasite Biology | de Oliveira LS, Alborghetti MR, ..., Charneau S | 10.3389/fcimb.2021.708834 |
| 24779470 | Assessment of phosphorylation in Toxoplasma glideosome assembly and function | Jacot D, Frenal K, ..., Soldati-Favre D | 10.1111/cmi.12307 |
| 18315523 | Toxoplasma gondii Hsp20 is a stripe-arranged chaperone-like protein associatedwith the outer leaflet of the inner membrane complex | de Miguel N, Lebrun M, ..., Angel SO | 10.1042/BC20080004 |
| 23025827 | The Plasmodium falciparum schizont phosphoproteome reveals extensivephosphatidylinositol and cAMP-protein kinase A signaling | Lasonder E, Green JL, ..., Alano P | 10.1021/pr300557m |
| 19047362 | GAP45 phosphorylation controls assembly of the Toxoplasma myosin XIV complex | Gilk SD, Gaskins E, Ward GE, Beckers CJ | 10.1128/EC.00201-08 |