Product Description: cAMP-dependent protein kinase regulatory subunit
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.11 (cAMP-dependent protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005952 | cAMP-dependent protein kinase complex |
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0005634 | nucleus |
| Function | GO:0071889 | 14-3-3 protein binding |
| Function | GO:0003723 | RNA binding |
| Function | GO:0008603 | cAMP-dependent protein kinase regulator activity |
| Function | GO:0005515 | protein binding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.47 | 0.82 |
| Trophozoite | expressed | 0.74 | 0.61 |
| Schizont | expressed | 2.73 | 0.08 |
| Gametocyte | expressed | 0.97 | 0.50 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1223100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.399 | MFS: -2.62 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q7KQK0
"Best" AlphaFill ligand hit: N2P (pentane-1,5-diamine, Local RMSD=0.02) with 4OFG (Global RMSD=2.04)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.11 | cAMP-dependent protein kinase | ADPH89PKIIP20KN-93KT5720HA1077U-73122KT-5720Rp-cAMPnaproxenimatinib... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P00514 | cAMP-dependent protein kinase type I-alpha regulatory subunit | Bos taurus | OMA, OrthoDB, OrthoMCL BLAST | MLS000766286MLS000860011MLS001333136MLS001148197... |
| P31323 | cAMP-dependent protein kinase type II-beta regulatory subunit | Homo sapiens | OrthoDB | MLS000766286MLS000860011MLS001333136MLS001148197... |
Ortholog Group (OrthoMCL): OG6_100449
Most Similar Human Ortholog: C9JR00
TM-align score: 0.70 | RMSD: 2.92
Seq Identity: 0.32 | Length: 141 / 441
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000005249 | protein kinase cAMP-dependent type II regulatory subunit beta |
| ENSG00000108946 | protein kinase cAMP-dependent type I regulatory subunit alpha |
| ENSG00000114302 | protein kinase cAMP-dependent type II regulatory subunit alpha |
| ENSG00000188191 | protein kinase cAMP-dependent type I regulatory subunit beta |
| ENSG00000284096 | protein kinase cAMP-dependent type II regulatory subunit beta |
Protein Length: 441 | Molecular Weight (kDa): 50.833
Isoelectric Point: 7.36
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000595 | Cyclic nucleotide-binding domain |
| InterPro | IPR012198 | cAMP-dependent protein kinase regulatory subunit |
| InterPro | IPR018488 | Cyclic nucleotide-binding, conserved site |
| InterPro | IPR018490 | Cyclic nucleotide-binding-like |
| PFam | PF00027 | Cyclic nucleotide-binding domain |
| Superfamily | SSF51206 | Cyclic nucleotide-binding-like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 15 | 8 | 11 |
| disruptive | 4 | 15 | 9 | 27 |
| missense | 4 | 15 | 9 | 24 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 27 | 5 | 18 |
| disruptive | 9 | 35 | 27 | 51 |
| missense | 8 | 26 | 22 | 36 |
PlasmoDB Total SNPs: 99
Non-coding: 87 | Synonymous: 5 | Nonsynonymous: 7 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 18248092 | Plasmodium falciparum regulatory subunit of cAMP-dependent PKA and anion channelconductance. | Merckx A, Nivez MP, ..., Egee S | 10.1371/journal.ppat.0040019 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 28680058 | PfCDPK1 mediated signaling in erythrocytic stages of Plasmodium falciparum. | Kumar S, Kumar M, ..., Sharma P | 10.1038/s41467-017-00053-1 |
| 30794532 | Phosphodiesterase beta is the master regulator of cAMP signalling during malariaparasite invasion. | Flueck C, Drought LG, ..., Baker DA | 10.1371/journal.pbio.3000154 |
| 32817103 | Phosphorylation-Dependent Assembly of a 14-3-3 Mediated Signaling Complex during Red Blood Cell Invasion by Plasmodium falciparum Merozoites | More KR, Kaur I, ..., Chitnis CE | 10.1128/mBio.01287-20 |
| 33608523 | 20S proteasomes secreted by the malaria parasite promote its growth. | Dekel E, Yaffe D, ..., Regev-Rudzki N | 10.1038/s41467-021-21344-8 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 34063491 | Development of Phosphodiesterase-Protein-Kinase Complexes as Novel Targets forDiscovery of Inhibitors with Enhanced Specificity | Tulsian NK, Sin VJ, Koh HL, Anand GS | 10.3390/ijms22105242 |
| 36941487 | In silico evolutionary and structural analysis of cAMP response proteins (CARPs)from Leishmania major | Bhakta S, Bhattacharya A | 10.1007/s00203-023-03463-6 |
| 36431013 | Functional Insights into Protein Kinase A (PKA) Signaling from C. elegans | Sadeghian F, Castaneda PG, Amin MR, Cram EJ | 10.3390/life12111878 |
| 33819633 | The crystal structure of yeast regulatory subunit reveals key evolutionaryinsights into Protein Kinase A oligomerization | Bardeci NG, Tofolon E, ..., Moreno S | 10.1016/j.jsb.2021.107732 |
| 35199643 | Complex effects of kinase localization revealed by compartment-specificregulation of protein kinase A activity | LaCroix R, Lin B, Kang TY, Levchenko A | 10.7554/eLife.66869 |
| 29449124 | Investigating PKA-RII specificity using analogs of the PKA:AKAP peptide inhibitorSTAD-2 | Bendzunas NG, Dorfler S, ..., Herberg FW | 10.1016/j.bmc.2018.02.001 |