Product Description: DNA gyrase subunit A
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 5.6.2.2 (DNA topoisomerase (ATP-hydrolyzing));5.99.1.3 (Transferred entry: 5.6.2.2)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0009330 | DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex |
| Component | GO:0020011 | apicoplast |
| Function | GO:0005524 | ATP binding |
| Function | GO:0003677 | DNA binding |
| Function | GO:0003918 | DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity |
| Process | GO:0006265 | DNA topological change |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.26 | 0.87 |
| Trophozoite | not expressed | 0.70 | 0.61 |
| Schizont | not expressed | 0.60 | 0.65 |
| Gametocyte | not expressed | 0.36 | 0.79 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1223300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.119 | MFS: -3.116 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I0X3
"Best" AlphaFill ligand hit: EVP (etoposide, Local RMSD=0.01) with 5CDP (Global RMSD=1.74)
BRENDA EC Inhibitors:
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P0AES4 | DNA gyrase subunit A/B | Escherichia coli | OrthoMCL, OrthoMCL BLAST | NOVOBIOCINBenzimidazole urea analogue, 2Benzimidazole urea analogue, 3Benzimidazole urea analogue, 4... |
| P05653 | DNA gyrase subunit A/B | Bacillus subtilis | OrthoMCL, OrthoMCL BLAST | PD125275Ciprofloxacin |
| P9WG47 | DNA gyrase subunit A/B | OrthoMCL, OrthoMCL BLAST | NOVOBIOCINMOXIFLOXACINGATIFLOXACINACH-702 | |
| P0C1U9 | DNA topoisomerase 4 subunit A | Staphylococcus aureus | OrthoMCL BLAST | CHEMBL202753CHEMBL379022CHEMBL425234CHEMBL203851... |
| Q2FYS4 | DNA topoisomerase 4 subunit A/B | OrthoMCL BLAST | US9040542, 23 | |
| Q45066 | DNA topoisomerase 4 subunit A [V210L]/B | Bacillus subtilis | OrthoMCL BLAST | PD125275Ciprofloxacin |
| Q931S2 | DNA topoisomerase 4 subunit A | OrthoMCL BLAST | CHEMBL363725CHEMBL186870CHEMBL184489CHEMBL365638... | |
| P20831 | DNA gyrase subunit A/B | Staphylococcus aureus | OrthoMCL BLAST | NOVOBIOCINBenzimidazole carbamate analogue, 1Benzimidazole urea analogue, 2Benzimidazole urea analogue, 3... |
| P0AFI2 | DNA topoisomerase 4 subunit A | Escherichia coli | OrthoMCL BLAST | NOVOBIOCINBenzimidazole urea analogue, 2Benzimidazole urea analogue, 3Benzimidazole urea analogue, 4... |
| Q47245 | DNA gyrase subunit A | OrthoMCL BLAST | OFLOXACINMoxifl-oxacinCHEMBL4459270CHEMBL4470688... |
Protein Length: 1222 | Molecular Weight (kDa): 143.144
UniProt ID(s): Q8I0X3
PDB ID(s): None
Isoelectric Point: 9.87
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002205 | DNA topoisomerase, type IIA, subunit A/C-terminal |
| InterPro | IPR006691 | DNA gyrase/topoisomerase IV, subunit A, C-terminal repeat |
| InterPro | IPR013760 | DNA topoisomerase, type IIA-like domain superfamily |
| InterPro | IPR035516 | DNA gyrase/topoisomerase IV, subunit A, C-terminal |
| PFam | PF00521 | DNA topoisomerase, type IIA, subunit A/C-terminal |
| PFam | PF03989 | DNA gyrase/topoisomerase IV, subunit A, C-terminal repeat |
| Superfamily | SSF101904 | DNA gyrase/topoisomerase IV, subunit A, C-terminal |
| Superfamily | SSF56719 | DNA topoisomerase, type IIA-like domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 37 | 15 | 57 |
| disruptive | 13 | 72 | 48 | 125 |
| missense | 9 | 67 | 44 | 106 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 73 | 38 | 58 |
| disruptive | 30 | 173 | 94 | 219 |
| missense | 14 | 139 | 84 | 169 |
PlasmoDB Total SNPs: 99
Non-coding: 25 | Synonymous: 57 | Nonsynonymous: 17 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 17220464 | Molecular cloning of apicoplast-targeted Plasmodium falciparum DNA gyrase genes:unique intrinsic ATPase activity and ATP-independent dimerization of PfGyrBsubunit. | Dar MA, Sharma A, Mondal N, Dhar SK | 10.1128/EC.00357-06 |
| 17499371 | Nuclear gyrB encodes a functional subunit of the Plasmodium falciparum gyrasethat is involved in apicoplast DNA replication. | Raghu Ram EV, Kumar A, ..., Habib S | https://pubmed.ncbi.nlm.nih.gov/17499371/ |
| 22474358 | Gene selective mRNA cleavage inhibits the development of Plasmodium falciparum. | Augagneur Y, Wesolowski D, ..., Ben Mamoun C | 10.1073/pnas.1203516109 |
| 26566222 | Investigating the Roles of the C-Terminal Domain of Plasmodium falciparum GyrA. | Nagano S, Seki E, ..., Heddle JG | 10.1371/journal.pone.0142313 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 38349187 | Detection of resistance to macrolides and fluoroquinolones in Mycoplasmagenitalium by targeted next-generation sequencing | Chiribau CB, Schmedes S, ..., Crowe SR | 10.1128/spectrum.03845-23 |
| 38392303 | Friend or Foe: Protein Inhibitors of DNA Gyrase | Ruan S, Tu CH, Bourne CR | 10.3390/biology13020084 |
| 38396892 | Antibiotic Combination to Effectively Postpone or Inhibit the In Vitro Inductionand Selection of Levofloxacin-Resistant Mutants in Elizabethkingia anophelis | Lee CC, Lai CH, ..., Lin JN | 10.3390/ijms25042215 |