Product Description: phospholipid-transporting ATPase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 10
EC Numbers: 3.6.3.1 (Transferred entry: 7.6.2.1);7.6.2.1 (P-type phospholipid transporter)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005768 | endosome |
| Component | GO:0016021 | integral component of membrane |
| Component | GO:0016020 | membrane |
| Component | GO:0005886 | plasma membrane |
| Component | GO:0005802 | trans-Golgi network |
| Function | GO:0005524 | ATP binding |
| Function | GO:0140326 | ATPase-coupled intramembrane lipid transporter activity |
| Process | GO:0015917 | aminophospholipid transport |
| Process | GO:0006897 | endocytosis |
| Process | GO:0045332 | phospholipid translocation |
| Process | GO:0006890 | retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 2.53 | 0.13 |
| Trophozoite | expressed | 1.82 | 0.22 |
| Schizont | expressed | 2.14 | 0.14 |
| Gametocyte | expressed | 1.24 | 0.41 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1223400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 2 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.126 | MFS: -2.87 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1438300)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1438300)
Modification: Disrupted | RMgm-3932
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5I3
"Best" AlphaFill ligand hit: P5S (phosphatidyl serine, Local RMSD=0.04) with 6LKN (Global RMSD=5.45)
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101160
Most Similar Human Ortholog: A0A0A0MR22
TM-align score: 0.92 | RMSD: 2.76
Seq Identity: 0.34 | Length: 882 / 1618
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000054793 | ATPase phospholipid transporting 9A (putative) |
| ENSG00000166377 | ATPase phospholipid transporting 9B (putative) |
| ENSG00000274273 | ATPase phospholipid transporting 9B (putative) |
| ENSG00000282266 | ATPase phospholipid transporting 9B (putative) |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 21 | 65 | 40 | 83 |
| disruptive | 19 | 71 | 51 | 124 |
| missense | 10 | 46 | 38 | 90 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 26 | 134 | 66 | 93 |
| disruptive | 45 | 193 | 91 | 264 |
| missense | 15 | 121 | 60 | 187 |
PlasmoDB Total SNPs: 321
Non-coding: 223 | Synonymous: 68 | Nonsynonymous: 30 | Stop Codon: 0
Protein Length: 1618 | Molecular Weight (kDa): 190.446
UniProt ID(s): Q8I5I3
PDB ID(s): None
Isoelectric Point: 8.87
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001757 | P-type ATPase |
| InterPro | IPR006539 | P-type ATPase, subfamily IV |
| InterPro | IPR008250 | P-type ATPase, A domain superfamily |
| InterPro | IPR018303 | P-type ATPase, phosphorylation site |
| InterPro | IPR023298 | P-type ATPase, transmembrane domain superfamily |
| InterPro | IPR023299 | P-type ATPase, cytoplasmic domain N |
| InterPro | IPR032630 | P-type ATPase, C-terminal |
| InterPro | IPR032631 | P-type ATPase, N-terminal |
| InterPro | IPR036412 | HAD-like superfamily |
| PFam | PF00122 | P-type ATPase, N-terminal |
| PFam | PF13246 | P-type ATPase, C-terminal |
| PFam | PF16209 | |
| PFam | PF16212 | |
| Superfamily | SSF56784 | HAD-like superfamily |
| Superfamily | SSF81653 | P-type ATPase, A domain superfamily |
| Superfamily | SSF81660 | P-type ATPase, cytoplasmic domain N |
| Superfamily | SSF81665 | P-type ATPase, transmembrane domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 11152613 | Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes. | Krogh A, Larsson B, von Heijne G, Sonnhammer EL | 10.1006/jmbi.2000.4315 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |