About Gene List

PF3D7_1223800 (YHM2)

Genome location: Pf3D7_12_v3:971,186..972,341(+)

Genome classification: Core

Function and Localization

Product Description: citrate/oxoglutarate carrier protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005739 mitochondrion
Function GO:0005371 tricarboxylate secondary active transmembrane transporter activity
Process GO:0015742 alpha-ketoglutarate transport
Process GO:0006843 mitochondrial citrate transmembrane transport
Process GO:0006842 tricarboxylic acid transport

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite possibly expressed N/A N/A
Ring expressed 0.19 0.90
Trophozoite expressed 0.22 0.86
Schizont expressed 0.04 0.97
Gametocyte expressed 0.27 0.84

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1223800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.31 | #Insertions: 4

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1438700)

  • Relative Growth Rate: 0.99 ± 0.09
  • Confidence: 6.21

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1438700)

Modification: Disrupted | RMgm-3934

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I5H9

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_108609

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 3 9 5 11
disruptive 4 14 6 33
missense 2 13 6 28

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 5 21 9 16
disruptive 8 42 33 94
missense 6 31 26 56

PlasmoDB Total SNPs: 40

Non-coding: 27 | Synonymous: 8 | Nonsynonymous: 5 | Stop Codon: 0

Protein Information

Protein Length: 308 | Molecular Weight (kDa): 34.887

UniProt ID(s): Q8I5H9

PDB ID(s): None

Isoelectric Point: 9.89

Protein Domain Annotations:

Source Family ID Description
InterPro IPR018108 Mitochondrial substrate/solute carrier
InterPro IPR023395 Mitochondrial carrier domain superfamily
PFam PF00153 Mitochondrial substrate/solute carrier
Superfamily SSF103506 Mitochondrial carrier domain superfamily

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
34494883 A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. van Esveld SL, Meerstein-Kessel L, ..., Huynen MA 10.1128/mSphere.00614-21
28784321 Three mitochondrial transporters of Saccharomyces cerevisiae are essential forammonium fixation and lysine biosynthesis in synthetic minimal medium Scarcia P, Palmieri L, ..., Rottensteiner H 10.1016/j.ymgme.2017.07.004
20371607 Identification and functional characterization of a novel mitochondrial carrierfor citrate and oxoglutarate in Saccharomyces cerevisiae Castegna A, Scarcia P, ..., Palmieri F 10.1074/jbc.M109.097188
34320205 Utilization of ethanol for itaconic acid biosynthesis by engineered Saccharomycescerevisiae Xu Y, Li Z 10.1093/femsyr/foab043
38339147 Ctp1 and Yhm2: Two Mitochondrial Citrate Transporters to Support MetabolicFlexibility of Saccharomyces cerevisiae Assalve G, Lunetti P, Zara V, Ferramosca A 10.3390/ijms25031870
9742088 A novel DNA-binding protein bound to the mitochondrial inner membrane restoresthe null mutation of mitochondrial histone Abf2p in Saccharomyces cerevisiae Cho JH, Ha SJ, ..., Chae CB 10.1128/MCB.18.10.5712