Product Description: GTP cyclohydrolase 1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.5.4.16 (GTP cyclohydrolase I)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005634 | nucleus |
| Function | GO:0005525 | GTP binding |
| Function | GO:0003934 | GTP cyclohydrolase I activity |
| Function | GO:0008270 | zinc ion binding |
| Process | GO:0009396 | folic acid-containing compound biosynthetic process |
| Process | GO:0006730 | one-carbon metabolic process |
| Process | GO:0009410 | response to xenobiotic stimulus |
| Process | GO:0006729 | tetrahydrobiopterin biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.36 | 0.83 |
| Trophozoite | expressed | 1.51 | 0.31 |
| Schizont | expressed | 0.45 | 0.73 |
| Gametocyte | not expressed | 0.55 | 0.71 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1224000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.119 | #Insertions: 6
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1438900)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1438900)
Modification: Disrupted | RMgm-4612
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5H7
"Best" AlphaFill ligand hit: 3PO (triphosphate, Local RMSD=0.02) with 1WPL (Global RMSD=2.97)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.5.4.16 | GTP cyclohydrolase I | ATPADPGTPGDPCTPUTPKClGMPITPTTPEDTAdGTP... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101153
Most Similar Human Ortholog: P30793
TM-align score: 0.70 | RMSD: 2.56
Seq Identity: 0.31 | Length: 191 / 389
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000131979 | GTP cyclohydrolase 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 13 | 5 | 21 |
| disruptive | 11 | 32 | 11 | 41 |
| missense | 11 | 32 | 10 | 37 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 21 | 9 | 28 |
| disruptive | 24 | 56 | 29 | 86 |
| missense | 19 | 50 | 20 | 63 |
PlasmoDB Total SNPs: 75
Non-coding: 52 | Synonymous: 11 | Nonsynonymous: 12 | Stop Codon: 0
Protein Length: 389 | Molecular Weight (kDa): 45.877
PDB ID(s): None
Isoelectric Point: 9.89
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001474 | GTP cyclohydrolase I |
| InterPro | IPR020602 | GTP cyclohydrolase I domain |
| PFam | PF01227 | GTP cyclohydrolase I domain |
| Superfamily | SSF55620 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 24745605 | Biochemical and functional characterization of Plasmodium falciparum GTPcyclohydrolase I. | Kumpornsin K, Kotanan N, ..., Chookajorn T | 10.1186/1475-2875-13-150 |
| 30265714 | Amplification of GTP-cyclohydrolase 1 gene in Plasmodium falciparum isolates withthe quadruple mutant of dihydrofolate reductase and dihydropteroate synthasegenes in Ghana. | Osei M, Ansah F, ..., Duah NO | 10.1371/journal.pone.0204871 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |