PF3D7_1224200

Genome location: Pf3D7_12_v3:981,063..984,811(+)

Genome classification: Core

Function and Localization

Product Description: BRO1 domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	not expressed	0.15	0.93
Trophozoite	not expressed	0.05	0.96
Schizont	expressed	0.33	0.82
Gametocyte	expressed	0.68	0.68

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1224200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.974 | MFS: -2.452 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1439100)

Relative Growth Rate: 0.91 ± 0.13
Confidence: 5.52

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1439100)

Modification: Disrupted | RMgm-3937

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I5H5

"Best" AlphaFill ligand hit: GSH (glutathione, Local RMSD=6.79) with 3QAW (Global RMSD=33.62)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533219

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	32	15	30
disruptive	7	29	17	94
missense	7	28	17	84

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	12	51	20	50
disruptive	15	75	61	158
missense	14	58	54	120

PlasmoDB Total SNPs: 186

Non-coding: 152 | Synonymous: 25 | Nonsynonymous: 9 | Stop Codon: 0

Protein Information

Protein Length: 819 | Molecular Weight (kDa): 98.715

UniProt ID(s): Q8I5H5

PDB ID(s): None

Isoelectric Point: 6.81

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004328	BRO1 domain
PFam	PF03097	BRO1 domain
Superfamily	N/A	N/A