Product Description: polyadenylate-binding protein 1, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0043186 | P granule |
| Component | GO:0009986 | cell surface |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Component | GO:0034399 | nuclear periphery |
| Component | GO:0005634 | nucleus |
| Component | GO:1990904 | ribonucleoprotein complex |
| Function | GO:0003723 | RNA binding |
| Function | GO:0003730 | mRNA 3'-UTR binding |
| Function | GO:0003729 | mRNA binding |
| Function | GO:0008143 | poly(A) binding |
| Function | GO:0008266 | poly(U) RNA binding |
| Process | GO:0006396 | RNA processing |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.60 | 0.36 |
| Trophozoite | expressed | 1.15 | 0.44 |
| Schizont | expressed | 0.76 | 0.58 |
| Gametocyte | expressed | 0.53 | 0.71 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1224300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.149 | MFS: -2.725 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1439200)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5H4
"Best" AlphaFill ligand hit: GDP (guanosine-5'-diphosphate, Local RMSD=1.29) with 1H2T (Global RMSD=5.09)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P11940 | Polyadenylate-binding protein 1 | Homo sapiens | OrthoDB | MLS000030667MLS000074721MLS000074731MLS000029721... |
Ortholog Group (OrthoMCL): OG6_100425
Most Similar Human Ortholog: E5RGH3
TM-align score: 0.96 | RMSD: 1.09
Seq Identity: 0.71 | Length: 108 / 875
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000070756 | poly(A) binding protein cytoplasmic 1 |
| ENSG00000090621 | poly(A) binding protein cytoplasmic 4 |
| ENSG00000101104 | poly(A) binding protein cytoplasmic 1 like |
| ENSG00000151846 | poly(A) binding protein cytoplasmic 3 |
Protein Length: 875 | Molecular Weight (kDa): 97.23
PDB ID(s): None
Isoelectric Point: 9.31
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000504 | RNA recognition motif domain |
| InterPro | IPR002004 | Polyadenylate-binding protein/Hyperplastic disc protein |
| InterPro | IPR006515 | Polyadenylate binding protein, human types 1, 2, 3, 4 |
| InterPro | IPR035979 | RNA-binding domain superfamily |
| InterPro | IPR036053 | PABC (PABP) domain |
| PFam | PF00076 | RNA recognition motif domain |
| PFam | PF00658 | Polyadenylate-binding protein/Hyperplastic disc protein |
| Superfamily | SSF54928 | RNA-binding domain superfamily |
| Superfamily | SSF63570 | PABC (PABP) domain |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 41 | 25 | 33 |
| disruptive | 3 | 32 | 22 | 73 |
| missense | 3 | 28 | 20 | 67 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 18 | 74 | 33 | 39 |
| disruptive | 8 | 90 | 59 | 161 |
| missense | 6 | 69 | 45 | 104 |
PlasmoDB Total SNPs: 258
Non-coding: 234 | Synonymous: 16 | Nonsynonymous: 8 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 19751846 | Isolation and functional characterization of eIF4F components and poly(A)-bindingprotein from Plasmodium falciparum. | Tuteja R, Pradhan A | 10.1016/j.parint.2009.09.001 |
| 20169188 | Universal features of post-transcriptional gene regulation are critical forPlasmodium zygote development. | Mair GR, Lasonder E, ..., Waters AP | 10.1371/journal.ppat.1000767 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 27128092 | Interrogating the Plasmodium Sporozoite Surface: Identification ofSurface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP byMass Spectrometry-Based Proteomics. | Swearingen KE, Lindner SE, ..., Sinnis P | 10.1371/journal.ppat.1005606 |
| 29359180 | Nuclear, Cytosolic, and Surface-Localized Poly(A)-Binding Proteins of Plasmodiumyoelii | Minns AM, Hart KJ, ..., Lindner SE | 10.1128/mSphere.00435-17 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 36275617 | Distinct mRNA and protein interactomes highlight functional differentiation ofmajor eIF4F-like complexes from Trypanosoma brucei | Bezerra MJR, Moura DMN, ..., de Melo Neto OP | 10.3389/fmolb.2022.971811 |
| 38148574 | The Plasmodium falciparum CCCH zinc finger protein MD3 regulates malegametocytogenesis through its interaction with RNA-binding proteins | Farrukh A, Musabyimana JP, ..., Ngwa CJ | 10.1111/mmi.15215 |
| 34063193 | Characterization of the RNA-Binding Protein TcSgn1 in Trypanosoma cruzi | Oliveira C, Gerber AP, Goldenberg S, Alves LR | 10.3390/microorganisms9050986 |
| 34705820 | Identification of novel proteins and mRNAs differentially bound to the LeishmaniaPoly(A) Binding Proteins reveals a direct association between PABP1, theRNA-binding protein RBP23 and mRNAs encoding ribosomal proteins | Assis LA, Santos Filho MVC, ..., de Melo Neto OP | 10.1371/journal.pntd.0009899 |
| 32373547 | GCN2-Like Kinase Modulates Stress Granule Formation During Nutritional Stress inTrypanosoma cruzi | Malvezzi AM, Arico M, ..., Schenkman S | 10.3389/fcimb.2020.00149 |