Genome location: Pf3D7_12_v3:1,000,407..1,002,443(-)
Genome classification: Core
Product Description: cytochrome c heme lyase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 4.4.1.17 (Holocytochrome-c synthase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005743 | mitochondrial inner membrane |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0004408 | holocytochrome-c synthase activity |
| Process | GO:0018063 | cytochrome c-heme linkage |
| Process | GO:0055114 | oxidation-reduction process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.12 | 0.94 |
| Trophozoite | expressed | 0.18 | 0.90 |
| Schizont | expressed | 0.08 | 0.96 |
| Gametocyte | expressed | 0.28 | 0.83 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1224600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.125 | MFS: -3.069 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1439500)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5H1
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 4.4.1.17 | Holocytochrome-c synthase | heminDeuteroheminN-ethylmaleimideHolocytochrome c |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101849
Most Similar Human Ortholog: P53701
TM-align score: 0.76 | RMSD: 2.29
Seq Identity: 0.36 | Length: 192 / 231
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000004961 | holocytochrome c synthase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 13 | 7 | 13 |
| disruptive | 1 | 12 | 8 | 28 |
| missense | 1 | 11 | 7 | 25 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 21 | 23 | 18 |
| disruptive | 6 | 35 | 26 | 52 |
| missense | 5 | 27 | 24 | 37 |
PlasmoDB Total SNPs: 111
Non-coding: 89 | Synonymous: 16 | Nonsynonymous: 6 | Stop Codon: 0
Protein Length: 231 | Molecular Weight (kDa): 27.717
UniProt ID(s): Q8I5H1
PDB ID(s): None
Isoelectric Point: 8.31
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000511 | Cytochrome c/c1 haem-lyase |
| PFam | PF01265 | Cytochrome c/c1 haem-lyase |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 35454139 | Investigation of the Molecular Mechanisms of the Eukaryotic Cytochrome-cMaturation System | Silva AV, Firmino MO, ..., Paquete CM | 10.3390/biom12040549 |