Genome location: Pf3D7_12_v3:1,018,351..1,024,017(+)
Genome classification: Core
Product Description: isoleucine--tRNA ligase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 6.1.1.5 (Isoleucine--tRNA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Function | GO:0004822 | isoleucine-tRNA ligase activity |
| Process | GO:0006428 | isoleucyl-tRNA aminoacylation |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.14 | 0.93 |
| Trophozoite | expressed | 0.78 | 0.59 |
| Schizont | expressed | 0.10 | 0.94 |
| Gametocyte | expressed | 0.55 | 0.71 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1225100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.811 | MFS: -3.027 | #Insertions: 1
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1440000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1440000)
Modification: Disrupted | RMgm-3940
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5G6
"Best" AlphaFill ligand hit: GSP (5'-guanosine-diphosphate-monothiophosphate, Local RMSD=13.85) with 6UT3 (Global RMSD=22.57)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.1.1.5 | isoleucine-tRNA ligase | tRNATrypsinsperminechlorideNSC70422muciproinmupirocinSB-205952SB-203207Ile-ol-AMPdiphosphateFuranomycin... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P00956 | Isoleucine--tRNA ligase | Escherichia coli | OrthoMCL | CHEMBL38412CHEMBL42195CHEMBL42269CHEMBL40757... |
Ortholog Group (OrthoMCL): OG6_101646
Most Similar Human Ortholog: Q9NSE4
TM-align score: 0.79 | RMSD: 3.75
Seq Identity: 0.26 | Length: 876 / 1696
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000067704 | isoleucyl-tRNA synthetase 2, mitochondrial |
Protein Length: 1696 | Molecular Weight (kDa): 204.246
UniProt ID(s): Q8I5G6
PDB ID(s): None
Isoelectric Point: 9.86
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001412 | Aminoacyl-tRNA synthetase, class I, conserved site |
| InterPro | IPR002300 | Aminoacyl-tRNA synthetase, class Ia |
| InterPro | IPR009008 | Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain |
| InterPro | IPR009080 | Aminoacyl-tRNA synthetase, class Ia, anticodon-binding |
| InterPro | IPR013155 | Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding |
| InterPro | IPR033708 | Isoleucyl tRNA synthetase type 1, anticodon-binding domain |
| PFam | PF00133 | Aminoacyl-tRNA synthetase, class Ia |
| PFam | PF08264 | Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding |
| Superfamily | SSF47323 | Aminoacyl-tRNA synthetase, class Ia, anticodon-binding |
| Superfamily | SSF50677 | Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain |
| Superfamily | SSF52374 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 63 | 31 | 84 |
| disruptive | 35 | 150 | 101 | 247 |
| missense | 27 | 138 | 96 | 211 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 21 | 142 | 65 | 105 |
| disruptive | 74 | 427 | 217 | 479 |
| missense | 46 | 333 | 175 | 322 |
PlasmoDB Total SNPs: 163
Non-coding: 49 | Synonymous: 61 | Nonsynonymous: 52 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 21205898 | Validation of isoleucine utilization targets in Plasmodium falciparum. | Istvan ES, Dharia NV, ..., Goldberg DE | 10.1073/pnas.1011560108 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |