PF3D7_1225500

Genome location: Pf3D7_12_v3:1,033,173..1,035,379(+)

Genome classification: Core

Function and Localization

Product Description: small subunit rRNA processing factor, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005730	nucleolus
Component	GO:0032040	small-subunit processome
Function	GO:0070037	rRNA (pseudouridine) methyltransferase activity
Function	GO:0019843	rRNA binding
Process	GO:0070475	rRNA base methylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.87	0.61
Trophozoite	expressed	0.51	0.73
Schizont	possibly expressed	0.11	0.93
Gametocyte	expressed	0.62	0.66

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1225500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 2 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.136 | MFS: -3.105 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1440400)

Relative Growth Rate: 0.38 ± 0.29
Confidence: 3.86

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1440400)

Modification: Disrupted | RMgm-3942

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I5G2

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_137661

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	6	3	31
disruptive	8	39	30	58
missense	6	35	24	54

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	22	17	31
disruptive	18	102	50	119
missense	10	78	47	92

PlasmoDB Total SNPs: 91

Non-coding: 65 | Synonymous: 14 | Nonsynonymous: 12 | Stop Codon: 0

Protein Information

Protein Length: 443 | Molecular Weight (kDa): 52.53

UniProt ID(s): Q8I5G2

PDB ID(s): None

Isoelectric Point: 10.12

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR005304	Ribosomal biogenesis, methyltransferase, EMG1/NEP1
InterPro	IPR029028	Alpha/beta knot methyltransferases
PFam	PF03587	Ribosomal biogenesis, methyltransferase, EMG1/NEP1
Superfamily	SSF75217	Alpha/beta knot methyltransferases

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104