About Gene List

PF3D7_1225700

Genome location: Pf3D7_12_v3:1,038,977..1,044,259(-)

Genome classification: Core

Function and Localization

Product Description: VAC14 domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0070772 PAS complex
Component GO:0010008 endosome membrane
Component GO:0000306 extrinsic component of vacuolar membrane
Component GO:0005774 vacuolar membrane
Process GO:0006661 phosphatidylinositol biosynthetic process
Process GO:0033674 positive regulation of kinase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring possibly expressed 0.70 0.68
Trophozoite possibly expressed 0.93 0.52
Schizont possibly expressed 0.18 0.89
Gametocyte not expressed 0.42 0.77

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1225700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.124 | MFS: -3.115 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1440600)

  • Relative Growth Rate: 0.22 ± 0.52
  • Confidence: 2.69

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I5G0

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102134

Most Similar Human Ortholog: H3BUU8

TM-align score: 0.87 | RMSD: 2.06

Seq Identity: 0.33 | Length: 196 / 1501

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000103043 VAC14 component of PIKFYVE complex

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 8 58 37 72
disruptive 39 154 75 203
missense 19 120 67 161

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 16 119 59 83
disruptive 82 327 142 322
missense 42 244 117 233

PlasmoDB Total SNPs: 198

Non-coding: 45 | Synonymous: 102 | Nonsynonymous: 51 | Stop Codon: 0

Protein Information

Protein Length: 1501 | Molecular Weight (kDa): 178.074

UniProt ID(s): Q8I5G0

PDB ID(s): None

Isoelectric Point: 5.23

Protein Domain Annotations:

Source Family ID Description
InterPro IPR016024 Armadillo-type fold
InterPro IPR021841 Vacuolar protein 14, C-terminal Fig4-binding domain
InterPro IPR026825 Vacuole morphology and inheritance protein 14
PFam PF11916 Vacuolar protein 14, C-terminal Fig4-binding domain
PFam PF12755
Superfamily SSF48371 Armadillo-type fold

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
12368868 Sequence of Plasmodium falciparum chromosomes 2, 10, 11 and 14. Gardner MJ, Shallom SJ, ..., Fraser CM 10.1038/nature01094
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104