PF3D7_1226100 (HAD3)

Genome location: Pf3D7_12_v3:1,057,390..1,059,654(+)

Genome classification: Core

Function and Localization

Product Description: haloacid dehalogenase-like hydrolase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.3.1 (Alkaline phosphatase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005829	cytosol
Function	GO:0003824	catalytic activity
Function	GO:0016787	hydrolase activity
Function	GO:0000287	magnesium ion binding
Function	GO:0016791	phosphatase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.02	0.99
Trophozoite	possibly expressed	0.05	0.97
Schizont	not expressed	0.03	0.98
Gametocyte	expressed	0.39	0.77

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1226100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.134 | MFS: -2.761 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1441100)

Relative Growth Rate: 0.92 ± 0.18
Confidence: 4.77

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1441000)

Modification: Disrupted | RMgm-3945

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I5F6

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.02) with 4N57 (Global RMSD=15.45)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.3.1	alkaline phosphatase	BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101515

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	10	7	15
disruptive	10	22	13	30
missense	10	22	12	30

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	1	26	9	24
disruptive	12	53	23	75
missense	12	50	20	50

PlasmoDB Total SNPs: 66

Non-coding: 44 | Synonymous: 5 | Nonsynonymous: 17 | Stop Codon: 0

Protein Information

Protein Length: 316 | Molecular Weight (kDa): 36.224

UniProt ID(s): Q8I5F6

PDB ID(s): None

Isoelectric Point: 6.5

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006379	HAD-superfamily hydrolase, subfamily IIB
InterPro	IPR036412	HAD-like superfamily
PFam	PF08282	N/A
Superfamily	SSF56784	HAD-like superfamily