Genome location: Pf3D7_12_v3:1,064,286..1,066,800(+)
Genome classification: Core
Product Description: haloacid dehalogenase-like hydrolase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB): None
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.41 | 0.79 |
| Trophozoite | expressed | 0.49 | 0.70 |
| Schizont | expressed | 0.23 | 0.84 |
| Gametocyte | possibly expressed | 0.46 | 0.74 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1226300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.959 | MFS: -2.598 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1441100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1441100)
Modification: Disrupted | RMgm-3946
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5F4
"Best" AlphaFill ligand hit: G3H (glyceraldehyde-3-phosphate, Local RMSD=1.31) with 4ZEX (Global RMSD=2.12)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 10 | 4 | 16 |
| disruptive | 2 | 0 | 1 | 14 |
| missense | 2 | 0 | 1 | 10 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 24 | 10 | 18 |
| disruptive | 5 | 21 | 18 | 52 |
| missense | 2 | 14 | 10 | 34 |
PlasmoDB Total SNPs: 101
Non-coding: 91 | Synonymous: 10 | Nonsynonymous: 0 | Stop Codon: 0
Protein Length: 295 | Molecular Weight (kDa): 33.268
UniProt ID(s): Q8I5F4
PDB ID(s): None
Isoelectric Point: 7.17
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000150 | Cof family |
| InterPro | IPR006379 | HAD-superfamily hydrolase, subfamily IIB |
| InterPro | IPR036412 | HAD-like superfamily |
| PFam | PF08282 | N/A |
| Superfamily | SSF56784 | HAD-like superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 30425143 | Suppression of Drug Resistance Reveals a Genetic Mechanism of MetabolicPlasticity in Malaria Parasites | Guggisberg AM, Frasse PM, ..., Odom John AR | 10.1128/mBio.01193-18 |
| 31205418 | Haloacid Dehalogenase Proteins: Novel Mediators of Metabolic Plasticity inPlasmodium falciparum | Frasse PM, Odom John AR | 10.1177/1178636119848435 |