Genome location: Pf3D7_12_v3:1,077,568..1,079,040(-)
Genome classification: Core
Product Description: proliferating cell nuclear antigen 2
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0043626 | PCNA complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0030337 | DNA polymerase processivity factor activity |
| Process | GO:0006272 | leading strand elongation |
| Process | GO:0006298 | mismatch repair |
| Process | GO:0051259 | protein complex oligomerization |
| Process | GO:0019985 | translesion synthesis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.03 | 0.98 |
| Trophozoite | possibly expressed | 0.30 | 0.81 |
| Schizont | expressed | 0.17 | 0.88 |
| Gametocyte | not expressed | 0.14 | 0.91 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1226600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.131 | MFS: -3.303 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1441400)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1441400)
Modification: Tagged | RMgm-4600
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q7KQJ9
"Best" AlphaFill ligand hit: NEQ (n-ethylmaleimide, Local RMSD=0.25) with 3V62 (Global RMSD=4.39)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P12004 | Proliferating cell nuclear antigen | Homo sapiens | HOGENOM, OrthoDB | CHEMBL2348305CHEMBL2348283CHEMBL2348282CHEMBL2348304... |
Protein Length: 264 | Molecular Weight (kDa): 30.163
PDB ID(s): None
Isoelectric Point: 4.74
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000730 | Proliferating cell nuclear antigen, PCNA |
| InterPro | IPR022648 | Proliferating cell nuclear antigen, PCNA, N-terminal |
| InterPro | IPR022649 | Proliferating cell nuclear antigen, PCNA, C-terminal |
| InterPro | IPR022659 | Proliferating cell nuclear antigen, PCNA, conserved site |
| PFam | PF00705 | Proliferating cell nuclear antigen, PCNA, N-terminal |
| PFam | PF02747 | Proliferating cell nuclear antigen, PCNA, C-terminal |
| Superfamily | SSF55979 | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 6 | 4 | 16 |
| disruptive | 0 | 2 | 3 | 10 |
| missense | 0 | 2 | 3 | 9 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 20 | 10 | 24 |
| disruptive | 1 | 15 | 17 | 35 |
| missense | 0 | 10 | 14 | 27 |
PlasmoDB Total SNPs: 75
Non-coding: 69 | Synonymous: 5 | Nonsynonymous: 1 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 12413950 | Molecular characterization and expression of an alternate proliferating cellnuclear antigen homologue, PfPCNA2, in Plasmodium falciparum. | Patterson S, Whittle C, Robert C, Chakrabarti D | 10.1016/s0006-291x(02)02436-1 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 30865364 | Molecular characterization and expression profile of an alternate proliferatingcell nuclear antigen homolog PbPCNA2 in Plasmodium berghei | Pradhan S, Kalia I, ..., Dhar SK | 10.1002/iub.2036 |
| 25728088 | Similarities and differences between Arabidopsis PCNA1 and PCNA2 in complementingthe yeast DNA damage tolerance defect | Xue C, Liang K, ..., Xiao W | 10.1016/j.dnarep.2015.02.003 |
| 30052905 | Role of tyrosine residue (Y213) in nuclear retention of PCNA1 in human malariaparasite Plasmodium falciparum | Banu K, Mitra P, Subbarao N, Dhar SK | 10.1093/femsle/fny182 |