PF3D7_1226800 (ATX3)

Genome location: Pf3D7_12_v3:1,083,776..1,087,560(+)

Genome classification: Core

Function and Localization

Product Description: ataxin-3, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.4.19.12 (Ubiquitinyl hydrolase 1)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Function	GO:0004843	thiol-dependent deubiquitinase
Process	GO:0016579	protein deubiquitination

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	possibly expressed	0.05	0.98
Trophozoite	expressed	0.25	0.84
Schizont	expressed	0.23	0.84
Gametocyte	expressed	0.23	0.86

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1226800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.121 | MFS: -2.924 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1441600)

Relative Growth Rate: 0.80 ± 0.30
Confidence: 3.77

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1441600)

Modification: Disrupted | RMgm-3949

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I5F0

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.19.12	ubiquitinyl hydrolase 1	KClNEMDTTEDTAEGTARNAisiRNApeptidesAntibodyubiquitinLDN-574442-propenal...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104644

Most Similar Human Ortholog: G3V4F4

TM-align score: 0.85 | RMSD: 2.17

Seq Identity: 0.32 | Length: 151 / 381

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000066427	ataxin 3
ENSG00000123594	ataxin 3 like

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	14	14	24
disruptive	1	11	10	55
missense	1	11	9	47

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	3	29	19	24
disruptive	4	31	38	91
missense	4	28	27	62

PlasmoDB Total SNPs: 256

Non-coding: 240 | Synonymous: 14 | Nonsynonymous: 2 | Stop Codon: 0

Protein Information

Protein Length: 381 | Molecular Weight (kDa): 44.98

UniProt ID(s): Q8I5F0

PDB ID(s): None

Isoelectric Point: 5.66

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006155	Josephin domain
InterPro	IPR029071	Ubiquitin-like domain superfamily
InterPro	IPR033865	Machado-Joseph disease protein
PFam	PF02099	Josephin domain
Superfamily	SSF54236	Ubiquitin-like domain superfamily