Genome location: Pf3D7_12_v3:1,092,884..1,096,419(-)
Genome classification: Core
Product Description: DNA helicase 60
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.12 (DNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Function | GO:0033679 | 3'-5' DNA/RNA helicase activity |
| Function | GO:0033678 | 5'-3' DNA/RNA helicase activity |
| Function | GO:0005524 | ATP binding |
| Function | GO:0016887 | ATP hydrolysis activity |
| Function | GO:0003723 | RNA binding |
| Function | GO:0003724 | RNA helicase activity |
| Function | GO:0003729 | mRNA binding |
| Process | GO:0016070 | RNA metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.09 | 0.95 |
| Trophozoite | expressed | 0.42 | 0.75 |
| Schizont | expressed | 0.06 | 0.96 |
| Gametocyte | expressed | 0.24 | 0.85 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1227100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: 0.194 | #Insertions: 2
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5E7
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.03) with 3RRM (Global RMSD=6.99)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.12 | DNA helicase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 26 | 20 | 50 |
| disruptive | 45 | 105 | 53 | 131 |
| missense | 19 | 55 | 42 | 93 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 16 | 68 | 29 | 40 |
| disruptive | 75 | 193 | 96 | 206 |
| missense | 34 | 118 | 75 | 129 |
PlasmoDB Total SNPs: 181
Non-coding: 118 | Synonymous: 43 | Nonsynonymous: 20 | Stop Codon: 0
Protein Length: 742 | Molecular Weight (kDa): 87.02
PDB ID(s): None
Isoelectric Point: 8.12
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000629 | ATP-dependent RNA helicase DEAD-box, conserved site |
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR011545 | DEAD/DEAH box helicase domain |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00270 | DEAD/DEAH box helicase domain |
| PFam | PF00271 | Helicase, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16165232 | Plasmodium falciparum DNA helicase 60 is a schizont stage specific, bipolar anddual helicase stimulated by PKC phosphorylation. | Pradhan A, Chauhan VS, Tuteja R | https://pubmed.ncbi.nlm.nih.gov/16165232/ |
| 17378213 | 'DEAD-box' helicase from Plasmodium falciparum is active at wide pH and isschizont stage-specific. | Pradhan A, Chauhan VS, Tuteja R | https://pubmed.ncbi.nlm.nih.gov/17378213/ |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 27381095 | The mRNA-bound proteome of the human malaria parasite Plasmodium falciparum. | Bunnik EM, Batugedara G, ..., Le Roch KG | 10.1186/s13059-016-1014-0 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 15694486 | A novel 'DEAD-box' DNA helicase from Plasmodium falciparum is homologous to p68 | Pradhan A, Chauhan VS, Tuteja R | 10.1016/j.molbiopara.2004.12.004 |
| 23916206 | Selection of antimalarial drug resistance after intermittent preventive treatmentof infants and children (IPTi/c) in Senegal | Ndiaye M, Tine R, ..., Gaye O | 10.1016/j.crvi.2013.04.016 |