Genome location: Pf3D7_12_v3:1,133,799..1,137,718(-)
Genome classification: Core
Product Description: elongator complex protein 3, putative
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 2.3.1.48 (Histone acetyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0033588 | elongator holoenzyme complex |
| Component | GO:0005741 | mitochondrial outer membrane |
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005634 | nucleus |
| Function | GO:0004402 | histone acetyltransferase activity |
| Process | GO:0002926 | tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 1.38 | 0.41 |
| Trophozoite | expressed | 0.87 | 0.56 |
| Schizont | not expressed | 0.24 | 0.86 |
| Gametocyte | not expressed | 0.53 | 0.71 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1227800
Resistome Missense Mutations: D832N
Resistome Compounds with Missense Mutations: MMV665794
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.731 | MFS: -2.938 | #Insertions: 1
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1442500)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5E0
"Best" AlphaFill ligand hit: DCA (desulfo-coenzyme a, Local RMSD=0.04) with 6IA6 (Global RMSD=3.21)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.1.48 | histone acetyltransferase | CoADNARNABF1EDTAMB-3LTK14PU139A-485TTK21CPTH2CPTH6... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100741
Most Similar Human Ortholog: B4DKA4
TM-align score: 0.92 | RMSD: 1.84
Seq Identity: 0.33 | Length: 454 / 1092
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000134014 | elongator acetyltransferase complex subunit 3 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 49 | 22 | 71 |
| disruptive | 27 | 72 | 56 | 118 |
| missense | 22 | 69 | 55 | 107 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 25 | 94 | 52 | 59 |
| disruptive | 44 | 164 | 89 | 179 |
| missense | 33 | 141 | 77 | 140 |
PlasmoDB Total SNPs: 99
Non-coding: 33 | Synonymous: 38 | Nonsynonymous: 28 | Stop Codon: 0
Protein Length: 1092 | Molecular Weight (kDa): 129.197
UniProt ID(s): Q8I5E0
PDB ID(s): None
Isoelectric Point: 8.29
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006638 | Elp3/MiaB/NifB |
| InterPro | IPR007197 | Radical SAM |
| InterPro | IPR032432 | Radical SAM, C-terminal extension |
| InterPro | IPR034687 | Elongator complex protein 3-like |
| InterPro | IPR039661 | ELP3/YhcC |
| PFam | PF04055 | Radical SAM |
| PFam | PF16199 | Radical SAM, C-terminal extension |
| Superfamily | SSF102114 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 23878194 | Elongator protein 3 (Elp3) lysine acetyltransferase is a tail-anchoredmitochondrial protein in Toxoplasma gondii. | Stilger KL, Sullivan WJ Jr | 10.1074/jbc.M113.491373 |
| 29293520 | Elp3 and RlmN: A tale of two mitochondrial tail-anchored radical SAM enzymes inToxoplasma gondii. | Padgett LR, Lentini JM, ..., Sullivan WJ Jr | 10.1371/journal.pone.0189688 |