PF3D7_1227900 (RPUSP)

Genome location: Pf3D7_12_v3:1,138,391..1,140,356(+)

Genome classification: Core

Function and Localization

Product Description: RNA pseudouridylate synthase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 4.2.1.70 (Pseudouridylate synthase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Process	GO:0009451	RNA modification

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.51	0.76
Trophozoite	not expressed	0.57	0.68
Schizont	not expressed	0.08	0.95
Gametocyte	not expressed	0.61	0.66

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1227900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.127 | MFS: -2.986 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1443200)

Relative Growth Rate: 0.02 ± 0.05
Confidence: 7.23

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I5D9

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
4.2.1.70	pseudouridylate synthase	tRNAiodoacetamide5-fluorouracilthiol reagents1,10-phenanthrolinep-chloromercuribenzoate1-(Tetrahydro-2-furanyl)-5-fluorouracil

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102367

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	18	13	29
disruptive	18	53	26	78
missense	13	43	21	68

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	41	21	28
disruptive	31	120	61	116
missense	19	92	51	86

PlasmoDB Total SNPs: 98

Non-coding: 19 | Synonymous: 33 | Nonsynonymous: 44 | Stop Codon: 2

Protein Information

Protein Length: 564 | Molecular Weight (kDa): 68.478

UniProt ID(s): Q8I5D9

PDB ID(s): None

Isoelectric Point: 9.25

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006145	Pseudouridine synthase, RsuA/RluA
InterPro	IPR020103	Pseudouridine synthase, catalytic domain superfamily
PFam	PF00849	Pseudouridine synthase, RsuA/RluA
Superfamily	SSF55120	Pseudouridine synthase, catalytic domain superfamily