Genome location: Pf3D7_12_v3:1,147,010..1,152,807(+)
Genome classification: Core
Product Description: NIMA related kinase 1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Process | GO:0000278 | mitotic cell cycle |
| Process | GO:0006468 | protein phosphorylation |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.05 | 0.98 |
| Trophozoite | expressed | 0.45 | 0.73 |
| Schizont | expressed | 0.45 | 0.71 |
| Gametocyte | expressed | 0.42 | 0.76 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1228300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.806 | MFS: -2.349 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1443000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5D5
"Best" AlphaFill ligand hit: B49 (sunitinib, Local RMSD=0.45) with 6NFY (Global RMSD=5.09)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.1 | non-specific serine/threonine protein kinase | ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_104455
Most Similar Human Ortholog: F6U4U2
TM-align score: 0.76 | RMSD: 3.67
Seq Identity: 0.34 | Length: 339 / 1057
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000117650 | NIMA related kinase 2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 25 | 69 | 23 | 66 |
| disruptive | 56 | 110 | 63 | 129 |
| missense | 21 | 56 | 43 | 101 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 40 | 132 | 44 | 66 |
| disruptive | 83 | 219 | 113 | 221 |
| missense | 34 | 124 | 81 | 142 |
PlasmoDB Total SNPs: 350
Non-coding: 223 | Synonymous: 63 | Nonsynonymous: 64 | Stop Codon: 0
Protein Length: 1057 | Molecular Weight (kDa): 124.945
PDB ID(s): None
Isoelectric Point: 9.82
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR008271 | Serine/threonine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| PFam | PF00069 | Protein kinase domain |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 21757488 | Plasmodium falciparum NIMA-related kinase Pfnek-1: sex specificity and assessmentof essentiality for the erythrocytic asexual cycle. | Dorin-Semblat D, Schmitt S, ..., Doerig C | https://pubmed.ncbi.nlm.nih.gov/21757488/ |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 23462523 | Nima- and Aurora-related kinases of malaria parasites. | Carvalho TG, Doerig C, Reininger L | 10.1016/j.bbapap.2013.02.022 |
| 33219324 | Human Aurora kinase inhibitor Hesperadin reveals epistatic interaction betweenPlasmodium falciparum PfArk1 and PfNek1 kinases. | Morahan BJ, Abrie C, ..., Garcia-Bustos JF | 10.1038/s42003-020-01424-z |