Product Description: RNA pseudouridylate synthase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 5.4.99.12 (tRNA pseudouridine(38-40) synthase)
Curated GO (PlasmoDB): None
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
Stage | LR class | MCA mean | MCA prop. zeros |
---|---|---|---|
Sporozoite | not expressed | N/A | N/A |
Ring | not expressed | 0.06 | 0.97 |
Trophozoite | expressed | 0.46 | 0.73 |
Schizont | possibly expressed | 0.53 | 0.68 |
Gametocyte | not expressed | 0.22 | 0.87 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1228500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.134 | MFS: -3.241 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1443200)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5D3
"Best" AlphaFill ligand hit: RHQ (rhodamine 6g, Local RMSD=26.03) with 3BR5 (Global RMSD=26.48)
BRENDA EC Inhibitors:
EC # | Name | EC Inhibitors |
---|---|---|
5.4.99.12 | tRNA pseudouridine38-40 synthase | tRNApoly(rA)npoly(rC)npoly(rG)npoly(rI)npoly(rU)niodoacetateCalf thymus DNADithionitrobenzoate5-fluorouracil tRNAPhep-chloromercuribenzoatefluorouracil-substituted tRNA |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 3 | 15 | 11 | 32 |
disruptive | 2 | 23 | 13 | 54 |
missense | 2 | 22 | 13 | 49 |
Any inclusion in genotype call
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 6 | 33 | 16 | 27 |
disruptive | 8 | 62 | 33 | 75 |
missense | 4 | 54 | 28 | 57 |
PlasmoDB Total SNPs: 130
Non-coding: 107 | Synonymous: 19 | Nonsynonymous: 4 | Stop Codon: 0
Protein Length: 409 | Molecular Weight (kDa): 48.366
UniProt ID(s): Q8I5D3
PDB ID(s): None
Isoelectric Point: 10.07
Protein Domain Annotations:
Source | Family ID | Description |
---|---|---|
InterPro | IPR002942 | RNA-binding S4 domain |
InterPro | IPR020103 | Pseudouridine synthase, catalytic domain superfamily |
PFam | PF01479 | RNA-binding S4 domain |
Superfamily | SSF55120 | Pseudouridine synthase, catalytic domain superfamily |
Superfamily | SSF55174 |
PMID | Title | Authors | DOI/Link |
---|---|---|---|
12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |