Genome location: Pf3D7_12_v3:1,208,198..1,209,870(+)
Genome classification: Core
Product Description: macrophage migration inhibitory factor
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 5.3.2.1 (Phenylpyruvate tautomerase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0030430 | host cell cytoplasm |
| Function | GO:0005125 | cytokine activity |
| Function | GO:0004536 | deoxyribonuclease activity |
| Function | GO:0046789 | host cell surface receptor binding |
| Function | GO:0016491 | oxidoreductase activity |
| Process | GO:0042783 | evasion of host immune response |
| Process | GO:0052170 | suppression by symbiont of host innate immune response |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.21 | 0.90 |
| Trophozoite | expressed | 0.57 | 0.68 |
| Schizont | expressed | 0.08 | 0.94 |
| Gametocyte | expressed | 0.11 | 0.94 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1229400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.996 | MFS: 0 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1444000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1444000)
Modification: Disrupted | RMgm-3967
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5C5
"Best" AlphaFill ligand hit: HBX (benzaldehyde, Local RMSD=0.02) with 4PLU (Global RMSD=2.32)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 5.3.2.1 | phenylpyruvate tautomerase | BRENDA SOAP query unsuccessful |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q8I5C5 | Macrophage migration inhibitory factor | Plasmodium falciparum | HOGENOM, OMA, OrthoDB, OrthoMCL, OrthoMCL BLAST | EpoxyazadiradioneCHEMBL2178862CHEMBL2178861CHEMBL2178858... |
| P14174 | Macrophage migration inhibitory factor | Homo sapiens | OrthoMCL BLAST | EpoxyazadiradioneBTB 09588, 1DFP 00129, 2DP 00477, 3... |
| Q1HEA2 | Macrophage migration inhibitory factor-like protein | OrthoMCL BLAST | Epoxyazadiradione | |
| P34884 | Macrophage migration inhibitory factor | Mus musculus | OrthoMCL BLAST | US8618147, 3US8618147, 8US8618147, 11US8618147, 12... |
Ortholog Group (OrthoMCL): OG6_104199
Most Similar Human Ortholog: P14174
TM-align score: 0.92 | RMSD: 1.52
Seq Identity: 0.29 | Length: 115 / 116
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000240972 | macrophage migration inhibitory factor |
| ENSG00000276701 | macrophage migration inhibitory factor |
Protein Length: 116 | Molecular Weight (kDa): 12.845
PDB ID(s): 2WKF
Isoelectric Point: 6.19
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001398 | Macrophage migration inhibitory factor |
| InterPro | IPR014347 | Tautomerase/MIF superfamily |
| PFam | PF01187 | Macrophage migration inhibitory factor |
| Superfamily | SSF55331 | Tautomerase/MIF superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 5 | 1 | 5 |
| disruptive | 2 | 3 | 3 | 9 |
| missense | 2 | 3 | 3 | 7 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 9 | 5 | 9 |
| disruptive | 3 | 13 | 7 | 27 |
| missense | 2 | 12 | 7 | 23 |
PlasmoDB Total SNPs: 97
Non-coding: 94 | Synonymous: 1 | Nonsynonymous: 2 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 17158894 | Functional characterization of the Plasmodium falciparum and P. bergheihomologues of macrophage migration inhibitory factor. | Augustijn KD, Kleemann R, ..., Waters AP | 10.1128/IAI.00902-06 |
| 17299722 | Characterization of a Plasmodium falciparum macrophage-migration inhibitoryfactor homologue. | Cordery DV, Kishore U, ..., Urban BC | 10.1086/511309 |
| 19811418 | Epitope mapping of monoclonal antibody 1B9 against plasmodium falciparum-derivedmacrophage migration inhibitory factor. | Wang Z, Shao D, ..., Wang H | 10.1080/08820130902912340 |
| 19827093 | The crystal structures of macrophage migration inhibitory factor from Plasmodiumfalciparum and Plasmodium berghei. | Dobson SE, Augustijn KD, ..., Wilkinson AJ | 10.1002/pro.263 |
| 34785370 | Characterization of Plasmodium falciparum macrophage migration inhibitory factorhomologue and its cysteine deficient mutants. | Schipper S, Springer E, ..., Przyborski JM | 10.1016/j.parint.2021.102513 |