Genome location: Pf3D7_12_v3:1,210,828..1,214,240(+)
Genome classification: Core
Product Description: T-complex protein 1 subunit gamma
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0036396 | RNA N6-methyladenosine methyltransferase complex |
| Component | GO:0005832 | chaperonin-containing T-complex |
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
| Function | GO:0005524 | ATP binding |
| Function | GO:0051082 | unfolded protein binding |
| Process | GO:0006457 | protein folding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.67 | 0.69 |
| Trophozoite | expressed | 1.03 | 0.49 |
| Schizont | expressed | 0.11 | 0.93 |
| Gametocyte | expressed | 0.56 | 0.70 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1229500
Resistome Missense Mutations: R336K
Resistome Compounds with Missense Mutations: 3-Hydroxypropanamidine 22
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.151 | MFS: -2.822 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1444100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5C4
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=1.06) with 1Q3S (Global RMSD=2.14)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100235
Most Similar Human Ortholog: E9PQ35
TM-align score: 0.97 | RMSD: 0.85
Seq Identity: 0.55 | Length: 144 / 542
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000150753 | chaperonin containing TCP1 subunit 5 |
| ENSG00000163468 | chaperonin containing TCP1 subunit 3 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 32 | 11 | 22 |
| disruptive | 1 | 7 | 7 | 31 |
| missense | 1 | 7 | 6 | 27 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 52 | 17 | 34 |
| disruptive | 3 | 35 | 25 | 80 |
| missense | 1 | 26 | 21 | 54 |
PlasmoDB Total SNPs: 174
Non-coding: 164 | Synonymous: 9 | Nonsynonymous: 1 | Stop Codon: 0
Protein Length: 542 | Molecular Weight (kDa): 60.662
UniProt ID(s): Q8I5C4
PDB ID(s): None
Isoelectric Point: 7.03
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002194 | Chaperonin TCP-1, conserved site |
| InterPro | IPR002423 | Chaperonin Cpn60/TCP-1 family |
| InterPro | IPR012719 | T-complex protein 1, gamma subunit |
| InterPro | IPR017998 | Chaperone tailless complex polypeptide 1 (TCP-1) |
| InterPro | IPR027409 | GroEL-like apical domain superfamily |
| InterPro | IPR027410 | TCP-1-like chaperonin intermediate domain superfamily |
| InterPro | IPR027413 | GroEL-like equatorial domain superfamily |
| PFam | PF00118 | Chaperonin Cpn60/TCP-1 family |
| Superfamily | SSF48592 | GroEL-like equatorial domain superfamily |
| Superfamily | SSF52029 | GroEL-like apical domain superfamily |
| Superfamily | SSF54849 | TCP-1-like chaperonin intermediate domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 25615740 | New Export Pathway in Plasmodium falciparum-Infected Erythrocytes: Role of theParasite Group II Chaperonin, PfTRiC. | Mbengue A, Vialla E, ..., Braun-Breton C | 10.1111/tra.12266 |
| 28067475 | The chaperonin T RiC forms an oligomeric complex in the malaria parasite cytosol | Spillman NJ, Beck JR, ..., Goldberg DE | 10.1111/cmi.12719 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 31384004 | Transcriptome-wide dynamics of extensive m(6)A mRNA methylation during Plasmodiumfalciparum blood-stage development. | Baumgarten S, Bryant JM, ..., Scherf A | 10.1038/s41564-019-0521-7 |
| 37193829 | Pathway and mechanism of tubulin folding mediated by TRiC/CCT along its ATPasecycle revealed using cryo-EM | Liu C, Jin M, ..., Cong Y | 10.1038/s42003-023-04915-x |