Genome location: Pf3D7_12_v3:1,218,464..1,227,615(-)
Genome classification: Core
Product Description: myosin J, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0015629 | actin cytoskeleton |
| Component | GO:0045178 | basal part of cell |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0031982 | vesicle |
| Function | GO:0051015 | actin filament binding |
| Function | GO:0030898 | actin-dependent ATPase activity |
| Function | GO:0000146 | microfilament motor activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0007015 | actin filament organization |
| Process | GO:0030050 | vesicle transport along actin filament |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.17 | 0.92 |
| Trophozoite | not expressed | 0.09 | 0.95 |
| Schizont | expressed | 1.71 | 0.45 |
| Gametocyte | expressed | 0.54 | 0.71 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1229800
Resistome Missense Mutations: L721F
Resistome Compounds with Missense Mutations: Suloctidil
Resistome # Samples with Disruptive Mutations: 5 (2 missense, 2 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.998 | MFS: -2.59 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1444500)
Modification: Tagged | RMgm-4664
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A144A488
"Best" AlphaFill ligand hit: P15 (2,5,8,11,14,17-hexaoxanonadecan-19-ol, Local RMSD=0.02) with 6QDJ (Global RMSD=4.35)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 34 | 92 | 54 | 153 |
| disruptive | 145 | 313 | 136 | 423 |
| missense | 75 | 248 | 113 | 327 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 52 | 195 | 95 | 148 |
| disruptive | 255 | 595 | 275 | 599 |
| missense | 133 | 460 | 204 | 385 |
PlasmoDB Total SNPs: 479
Non-coding: 198 | Synonymous: 157 | Nonsynonymous: 124 | Stop Codon: 0
Protein Length: 2274 | Molecular Weight (kDa): 270.781
UniProt ID(s): A0A144A488, Q967M4
PDB ID(s): None
Isoelectric Point: 8.44
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001609 | Myosin head, motor domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00063 | Myosin head, motor domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 31283102 | Systematic analysis of Plasmodium myosins reveals differential expression,localisation, and function in invasive and proliferative parasite stages | Wall RJ, Zeeshan M, ..., Tewari R | 10.1111/cmi.13082 |
| 36310866 | Identification of new components of the basal pole of Toxoplasma gondii providesnovel insights into its molecular organization and functions | Roumegous C, Abou Hammoud A, ..., Frenal K | 10.3389/fcimb.2022.1010038 |