PF3D7_1230500

Genome location: Pf3D7_12_v3:1,253,486..1,255,841(-)

Genome classification: Core

Function and Localization

Product Description: WD repeat-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0097344	Rix1 complex
Component	GO:0005656	nuclear pre-replicative complex
Process	GO:0006364	rRNA processing
Process	GO:0030174	regulation of DNA-dependent DNA replication initiation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.22	0.89
Trophozoite	expressed	0.18	0.89
Schizont	possibly expressed	0.02	0.99
Gametocyte	not expressed	0.22	0.87

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1230500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -3.108 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1445200)

Relative Growth Rate: 0.29 ± 0.26
Confidence: 4.08

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I5B5

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103008

Most Similar Human Ortholog: A0A087X237

TM-align score: 0.78 | RMSD: 2.57

Seq Identity: 0.12 | Length: 140 / 502

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000065268	WD repeat domain 18

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	15	9	28
disruptive	12	68	39	83
missense	5	59	36	72

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	9	37	15	24
disruptive	23	143	66	135
missense	10	118	55	94

PlasmoDB Total SNPs: 112

Non-coding: 64 | Synonymous: 35 | Nonsynonymous: 13 | Stop Codon: 0

Protein Information

Protein Length: 502 | Molecular Weight (kDa): 59.27

UniProt ID(s): Q8I5B5

PDB ID(s): None

Isoelectric Point: 5.97

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001680	WD40 repeat
InterPro	IPR036322	WD40-repeat-containing domain superfamily
PFam	N/A	N/A
Superfamily	SSF50978	WD40-repeat-containing domain superfamily