PF3D7_1230600 (NSUN4)

Genome location: Pf3D7_12_v3:1,256,554..1,258,824(+)

Genome classification: Core

Function and Localization

Product Description: RNA cytosine C(5)-methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.- (Methyltransferases.)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005730	nucleolus
Function	GO:0008168	methyltransferase activity
Function	GO:0009383	rRNA (cytosine-C5-)-methyltransferase activity
Process	GO:0001510	RNA methylation
Process	GO:0000470	maturation of LSU-rRNA
Process	GO:0070475	rRNA base methylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.36	0.82
Trophozoite	expressed	0.38	0.77
Schizont	expressed	0.15	0.90
Gametocyte	expressed	0.37	0.79

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1230600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.142 | MFS: -2.805 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1445300)

Relative Growth Rate: 0.10 ± 0.15
Confidence: 1.00

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I5B4

"Best" AlphaFill ligand hit: SFG (sinefungin, Local RMSD=3.42) with 2YXL (Global RMSD=4.09)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.-	Ex: homocysteine S-methyltransferase	EDTACTABNickelA9145CtylosintacrineTween 80FTI-2628macrocinadenosineRelomycinphosphate...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100487

Most Similar Human Ortholog: P46087

TM-align score: 0.77 | RMSD: 3.37

Seq Identity: 0.25 | Length: 323 / 376

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000111641	NOP2 nucleolar protein

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	10	17	16
disruptive	1	5	6	28
missense	1	5	3	23

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	46	13	12
disruptive	4	27	34	64
missense	2	18	23	39

PlasmoDB Total SNPs: 102

Non-coding: 97 | Synonymous: 5 | Nonsynonymous: 0 | Stop Codon: 0

Protein Information

Protein Length: 376 | Molecular Weight (kDa): 43.722

UniProt ID(s): Q8I5B4

PDB ID(s): None

Isoelectric Point: 9.84

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001678	SAM-dependent methyltransferase RsmB/NOP2-type
InterPro	IPR023267	RNA (C5-cytosine) methyltransferase
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
PFam	PF01189	SAM-dependent methyltransferase RsmB/NOP2-type
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase