Genome location: Pf3D7_12_v3:1,258,922..1,262,608(+)
Genome classification: Core
Product Description: protein transport protein SEC13
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0030127 | COPII vesicle coat |
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005829 | cytosol |
| Component | GO:0070971 | endoplasmic reticulum exit site |
| Component | GO:0005643 | nuclear pore |
| Component | GO:0031080 | nuclear pore outer ring |
| Component | GO:0005654 | nucleoplasm |
| Process | GO:0090114 | COPII-coated vesicle budding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.39 | 0.80 |
| Trophozoite | expressed | 0.35 | 0.78 |
| Schizont | expressed | 0.32 | 0.78 |
| Gametocyte | expressed | 0.20 | 0.88 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1230700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 2 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.989 | MFS: -1.951 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1445400)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_144540)
Modification: Mutated | RMgm-4474
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5B3
"Best" AlphaFill ligand hit: ALQ (2-methyl-propionic acid, Local RMSD=0.24) with 4GM3 (Global RMSD=4.39)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101941
Most Similar Human Ortholog: A8MXL6
TM-align score: 0.93 | RMSD: 2.03
Seq Identity: 0.36 | Length: 281 / 822
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000157020 | SEC13 homolog, nuclear pore and COPII coat complex component |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 15 | 40 | 12 | 46 |
| disruptive | 68 | 143 | 71 | 154 |
| missense | 22 | 93 | 58 | 120 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 25 | 65 | 29 | 53 |
| disruptive | 106 | 266 | 129 | 231 |
| missense | 43 | 192 | 98 | 133 |
PlasmoDB Total SNPs: 179
Non-coding: 73 | Synonymous: 59 | Nonsynonymous: 47 | Stop Codon: 0
Protein Length: 822 | Molecular Weight (kDa): 90.749
UniProt ID(s): Q8I5B3
PDB ID(s): None
Isoelectric Point: 6.58
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001680 | WD40 repeat |
| InterPro | IPR036322 | WD40-repeat-containing domain superfamily |
| InterPro | IPR037363 | Sec13/Seh1 family |
| InterPro | IPR037596 | Protein Sec13 |
| PFam | PF00400 | WD40 repeat |
| Superfamily | SSF50978 | WD40-repeat-containing domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 18284574 | Spatial dissection of the cis- and trans-Golgi compartments in the malariaparasite Plasmodium falciparum. | Struck NS, Herrmann S, ..., Gilberger TW | 10.1111/j.1365-2958.2008.06125.x |
| 23687383 | PfSec13 is an unusual chromatin-associated nucleoporin of Plasmodium falciparumthat is essential for parasite proliferation in human erythrocytes. | Dahan-Pasternak N, Nasereddin A, ..., Dzikowski R | 10.1242/jcs.122119 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |
| 35727050 | Stage-Specific COPII-Mediated Cargo Selectivity in African Trypanosomes | Sharif M, Bangs JD | 10.1128/msphere.00188-22 |