Genome location: Pf3D7_12_v3:1,263,243..1,266,561(+)
Genome classification: Core
Product Description: pre-mRNA-splicing regulator WTAP, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0036396 | RNA N6-methyladenosine methyltransferase complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0005515 | protein binding |
| Process | GO:0080009 | mRNA methylation |
| Process | GO:0000381 | regulation of alternative mRNA splicing, via spliceosome |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.94 | 0.57 |
| Trophozoite | expressed | 0.94 | 0.50 |
| Schizont | expressed | 0.25 | 0.85 |
| Gametocyte | expressed | 0.70 | 0.62 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1230800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.219 | MFS: -2.677 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5B2
"Best" AlphaFill ligand hit: GNP (phosphoaminophosphonic acid-guanylate ester, Local RMSD=0.11) with 3ZJC (Global RMSD=42.36)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_105437
Most Similar Human Ortholog: A0A087X1R4
TM-align score: 0.49 | RMSD: 2.74
Seq Identity: 0.14 | Length: 100 / 584
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000146457 | WT1 associated protein |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 17 | 6 | 30 |
| disruptive | 25 | 55 | 31 | 89 |
| missense | 8 | 31 | 23 | 68 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 17 | 25 | 19 | 34 |
| disruptive | 45 | 117 | 70 | 135 |
| missense | 13 | 78 | 50 | 80 |
PlasmoDB Total SNPs: 222
Non-coding: 164 | Synonymous: 41 | Nonsynonymous: 14 | Stop Codon: 3
Protein Length: 584 | Molecular Weight (kDa): 71.308
UniProt ID(s): Q8I5B2
PDB ID(s): None
Isoelectric Point: 8.15
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR029732 | WTAP/Mum2 family |
| InterPro | IPR033757 | Pre-mRNA-splicing regulator WTAP |
| PFam | PF17098 | WTAP/Mum2 family |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 31384004 | Transcriptome-wide dynamics of extensive m(6)A mRNA methylation during Plasmodiumfalciparum blood-stage development. | Baumgarten S, Bryant JM, ..., Scherf A | 10.1038/s41564-019-0521-7 |