Genome location: Pf3D7_12_v3:1,266,588..1,270,610(+)
Genome classification: Core
Product Description: serine/threonine protein kinase RIO1, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0004674 | protein serine/threonine kinase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.57 | 0.36 |
| Trophozoite | expressed | 0.77 | 0.60 |
| Schizont | expressed | 0.28 | 0.84 |
| Gametocyte | expressed | 0.52 | 0.72 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1230900
Resistome Missense Mutations: H522Y
Resistome Compounds with Missense Mutations: AN13762
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.248 | MFS: -2.75 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1445600)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1445600)
Modification: Disrupted | RMgm-3975
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A144A2N6
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.46) with 1ZTH (Global RMSD=4.64)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.1 | non-specific serine/threonine protein kinase | ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101068
Most Similar Human Ortholog: Q9BRS2
TM-align score: 0.49 | RMSD: 3.99
Seq Identity: 0.40 | Length: 312 / 937
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000124784 | RIO kinase 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 35 | 29 | 53 |
| disruptive | 32 | 56 | 60 | 172 |
| missense | 14 | 44 | 53 | 140 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 74 | 31 | 50 |
| disruptive | 52 | 177 | 123 | 214 |
| missense | 20 | 130 | 94 | 154 |
PlasmoDB Total SNPs: 184
Non-coding: 85 | Synonymous: 52 | Nonsynonymous: 45 | Stop Codon: 2
Protein Length: 937 | Molecular Weight (kDa): 111.471
UniProt ID(s): A0A144A2N6
PDB ID(s): None
Isoelectric Point: 6.33
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000687 | RIO kinase |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| InterPro | IPR018935 | RIO kinase, conserved site |
| PFam | PF01163 | N/A |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 24948609 | Dominant Rio1 kinase/ATPase catalytic mutant induces trapping of late pre-40Sbiogenesis factors in 80S-like ribosomes | Ferreira-Cerca S, Kiburu I, ..., Hurt E | 10.1093/nar/gku542 |
| 33908345 | The final step of 40S ribosomal subunit maturation is controlled by a dual keylock | Plassart L, Shayan R, ..., Plisson-Chastang C | 10.7554/eLife.61254 |
| 30011030 | Integrating Rio1 activities discloses its nutrient-activated network inSaccharomyces cerevisiae | Iacovella MG, Bremang M, ..., De Wulf P | 10.1093/nar/gky618 |
| 29237037 | Insights into the evolutionary conserved regulation of Rio ATPase activity | Knuppel R, Christensen RH, ..., Ferreira-Cerca S | 10.1093/nar/gkx1236 |
| 25294836 | Rio1 mediates ATP-dependent final maturation of 40S ribosomal subunits | Turowski TW, Lebaron S, ..., Tollervey D | 10.1093/nar/gku878 |
| 35031584 | The modifying enzyme Tsr3 establishes the hierarchy of Rio kinase binding in 40Sribosome assembly | Huang H, Parker M, Karbstein K | 10.1261/rna.078994.121 |
| 37263996 | Rio1 downregulates centromeric RNA levels to promote the timely assembly ofstructurally fit kinetochores | Smurova K, Damizia M, ..., De Wulf P | 10.1038/s41467-023-38920-9 |
| 25851096 | Rio1 promotes rDNA stability and downregulates RNA polymerase I to ensure rDNAsegregation | Iacovella MG, Golfieri C, ..., De Wulf P | 10.1038/ncomms7643 |
| 28977579 | The Rio1p ATPase hinders premature entry into translation of late pre-40Spre-ribosomal particles | Belhabich-Baumas K, Joret C, ..., Mougin A | 10.1093/nar/gkx734 |
| 28178206 | The Link between Protein Kinase CK2 and Atypical Kinase Rio1 | Kubinski K, Maslyk M | 10.3390/ph10010021 |
| 30515528 | The Rio1 protein kinases/ATPases: conserved regulators of growth, division, and genomic stability | Berto G, Ferreira-Cerca S, De Wulf P | 10.1007/s00294-018-0912-y |