Genome location: Pf3D7_12_v3:1,275,574..1,278,214(+)
Genome classification: Core
Product Description: ras-related protein Rab-2
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.5.2 (Small monomeric GTPase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005794 | Golgi apparatus |
| Component | GO:0020020 | food vacuole |
| Component | GO:0005773 | vacuole |
| Process | GO:0006888 | endoplasmic reticulum to Golgi vesicle-mediated transport |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.06 | 0.97 |
| Trophozoite | expressed | 0.11 | 0.92 |
| Schizont | expressed | 0.11 | 0.92 |
| Gametocyte | expressed | 0.06 | 0.97 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1231100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.165 | MFS: -3.157 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1445800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5A9
"Best" AlphaFill ligand hit: GNP (phosphoaminophosphonic acid-guanylate ester, Local RMSD=0.06) with 3LAW (Global RMSD=2.24)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.5.2 | small monomeric GTPase | GTPGDPEDTAPlexAstatinCCG-977Rap1GapNSC23766CCG-1423Rap1GAP1GTPgammaSCalmodulin... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P61105 | Ras-related protein Rab-2A | Canis lupus dingo | OrthoDB, OrthoMCL, OrthoMCL BLAST | MLS000095936MLS000088004MLS000119085MLS000544534... |
Ortholog Group (OrthoMCL): OG6_101535
Most Similar Human Ortholog: E9PE37
TM-align score: 0.96 | RMSD: 0.61
Seq Identity: 0.84 | Length: 62 / 213
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000104388 | RAB2A, member RAS oncogene family |
| ENSG00000129472 | RAB2B, member RAS oncogene family |
Protein Length: 213 | Molecular Weight (kDa): 24.424
PDB ID(s): None
Isoelectric Point: 6.71
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001806 | Small GTPase |
| InterPro | IPR005225 | Small GTP-binding protein domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00071 | Small GTPase |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 12 | 3 | 15 |
| disruptive | 0 | 1 | 2 | 7 |
| missense | 0 | 1 | 1 | 7 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 19 | 5 | 17 |
| disruptive | 0 | 9 | 12 | 43 |
| missense | 0 | 6 | 11 | 34 |
PlasmoDB Total SNPs: 81
Non-coding: 78 | Synonymous: 3 | Nonsynonymous: 0 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 12657463 | The Plasmodium falciparum family of Rab GTPases. | Quevillon E, Spielmann T, ..., Langsley G | 10.1016/s0378-1119(03)00381-0 |
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
| 37398139 | Toxoplasma gondii encodes an array of TBC-domain containing proteins including anessential regulator that targets Rab2 in the secretory pathway | Quan JJ, Nikolov LA, ..., Bradley PJ | 10.1101/2023.05.28.542599 |
| 34837645 | Golgi requires a new casting in the screenplay of mucopolysaccharidosis IIcytopathology | Molnar K, Kobolak J, Dinnyes A | 10.1007/s42977-021-00107-y |