Genome location: Pf3D7_12_v3:1,300,633..1,305,728(-)
Genome classification: Core
Product Description: pre-mRNA-splicing factor ATP-dependent RNA helicase PRP2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.13 (RNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005681 | spliceosomal complex |
| Function | GO:0008186 | ATP-dependent activity, acting on RNA |
| Function | GO:0003723 | RNA binding |
| Function | GO:0003724 | RNA helicase activity |
| Process | GO:0008380 | RNA splicing |
| Process | GO:0000398 | mRNA splicing, via spliceosome |
| Process | GO:0051726 | regulation of cell cycle |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.37 | 0.83 |
| Trophozoite | expressed | 0.88 | 0.54 |
| Schizont | expressed | 0.28 | 0.83 |
| Gametocyte | expressed | 0.61 | 0.67 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1231600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.47 | MFS: -2.544 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5A4
"Best" AlphaFill ligand hit: MPO (3[n-morpholino]propane sulfonic acid, Local RMSD=0.15) with 6ZM2 (Global RMSD=1.83)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.13 | RNA helicase | ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 18 | 45 | 38 | 58 |
| disruptive | 53 | 137 | 60 | 171 |
| missense | 20 | 64 | 39 | 117 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 31 | 98 | 39 | 55 |
| disruptive | 108 | 253 | 105 | 247 |
| missense | 35 | 141 | 67 | 170 |
PlasmoDB Total SNPs: 297
Non-coding: 140 | Synonymous: 99 | Nonsynonymous: 58 | Stop Codon: 0
Protein Length: 1168 | Molecular Weight (kDa): 137.197
UniProt ID(s): Q8I5A4
PDB ID(s): None
Isoelectric Point: 8.6
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR002464 | DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site |
| InterPro | IPR007502 | Helicase-associated domain |
| InterPro | IPR011545 | DEAD/DEAH box helicase domain |
| InterPro | IPR011709 | Domain of unknown function DUF1605 |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00270 | DEAD/DEAH box helicase domain |
| PFam | PF00271 | Helicase, C-terminal |
| PFam | PF04408 | Helicase-associated domain |
| PFam | PF07717 | Domain of unknown function DUF1605 |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 33243853 | Mechanism of spliceosome remodeling by the ATPase/helicase Prp2 and its coactivator Spp2 | Bai R, Wan R, ..., Shi Y | 10.1126/science.abe8863 |
| 34706246 | Cross talk between the upstream exon-intron junction and Prp2 facilitates splicing of non-consensus introns | Hummer S, Borao S, ..., Ayte J | 10.1016/j.celrep.2021.109893 |
| 34352092 | eIF4G is retained on ribosomes elongating and terminating on short upstream ORFsto control reinitiation in yeast | Mohammad MP, Smirnova A, Gunisova S, Valasek LS | 10.1093/nar/gkab652 |
| 33825710 | The structure of Prp2 bound to RNA and ADP-BeF(3)(-) reveals structural features important for RNA unwinding by DEAH-box ATPases | Hamann F, Zimmerningkat LC, ..., Ficner R | 10.1107/S2059798321001194 |
| 32553949 | Isolation, structure identification and anti-inflammatory activity of apolysaccharide from Phragmites rhizoma | Zhou R, Cui M, ..., Liu K | 10.1016/j.ijbiomac.2020.06.124 |
| 37780250 | Structure characterization of novel heteropolysaccharides from Pteridiumrevolutum with antioxidant and antiglycated activities | Wang KW, Sheng XY, ..., Wang SW | 10.1016/j.fochx.2023.100826 |
| 35932810 | Phragmites rhizoma polysaccharide-based nanocarriers for synergistic treatment ofulcerative colitis | Cui M, Fang Z, ..., Liu K | 10.1016/j.ijbiomac.2022.07.245 |
| 37548918 | Crystal structure of Prp16 in complex with ADP | Garbers TB, Enders M, Neumann P, Ficner R | 10.1107/S2053230X23005721 |