Genome location: Pf3D7_12_v3:1,325,076..1,328,093(-)
Genome classification: Core
Product Description: phenylalanine--tRNA ligase
SignalP Peptide: MFFSFFVCFIFVIIEFIES
# Transmembrane Domains: 0
EC Numbers: 6.1.1.20 (Phenylalanine--tRNA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005737 | cytoplasm |
| Function | GO:0004826 | phenylalanine-tRNA ligase activity |
| Function | GO:0070042 | rRNA (uridine-N3-)-methyltransferase activity |
| Process | GO:0006432 | phenylalanyl-tRNA aminoacylation |
| Process | GO:0070475 | rRNA base methylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.05 | 0.97 |
| Trophozoite | expressed | 0.48 | 0.71 |
| Schizont | possibly expressed | 0.13 | 0.90 |
| Gametocyte | not expressed | 0.27 | 0.84 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1232000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.221 | MFS: -2.709 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5A1
"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.52) with 3PCO (Global RMSD=2.91)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.1.1.20 | phenylalanine-tRNA ligase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 25 | 11 | 25 |
| disruptive | 7 | 34 | 24 | 77 |
| missense | 7 | 31 | 23 | 69 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 42 | 18 | 31 |
| disruptive | 22 | 89 | 68 | 109 |
| missense | 13 | 70 | 60 | 84 |
PlasmoDB Total SNPs: 83
Non-coding: 65 | Synonymous: 10 | Nonsynonymous: 8 | Stop Codon: 0
Protein Length: 556 | Molecular Weight (kDa): 67.628
UniProt ID(s): Q8I5A1
PDB ID(s): None
Isoelectric Point: 9.49
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002319 | Phenylalanyl-tRNA synthetase |
| InterPro | IPR004530 | Phenylalanyl-tRNA synthetase, class IIc, mitochondrial |
| InterPro | IPR005121 | Ferrodoxin-fold anticodon-binding domain |
| InterPro | IPR036690 | Ferrodoxin-fold anticodon-binding domain superfamily |
| PFam | PF01409 | Phenylalanyl-tRNA synthetase |
| Superfamily | SSF54991 | Ferrodoxin-fold anticodon-binding domain superfamily |
| Superfamily | SSF55681 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 25391660 | Plasmodium falciparum mitochondria import tRNAs along with an activephenylalanyl-tRNA synthetase. | Sharma A, Sharma A | 10.1042/BJ20140998 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |