Genome location: Pf3D7_12_v3:1,328,121..1,332,034(-)
Genome classification: Core
Product Description: 60 kDa chaperonin
SignalP Peptide: MRMKRIHILLLVIFLLCLRYGYS
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
Type | GO Term | Name |
---|---|---|
Component | GO:0020011 | apicoplast |
Component | GO:0020020 | food vacuole |
Component | GO:0005634 | nucleus |
Function | GO:0003723 | RNA binding |
Function | GO:0051082 | unfolded protein binding |
Process | GO:0006457 | protein folding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
Stage | LR class | MCA mean | MCA prop. zeros |
---|---|---|---|
Sporozoite | not expressed | N/A | N/A |
Ring | possibly expressed | 0.27 | 0.88 |
Trophozoite | expressed | 1.40 | 0.35 |
Schizont | expressed | 0.81 | 0.55 |
Gametocyte | expressed | 0.74 | 0.61 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1232100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.148 | MFS: -2.575 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1446800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I0V3
"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=1.72) with 7K3Z (Global RMSD=3.41)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 7 | 31 | 22 | 42 |
disruptive | 16 | 31 | 16 | 68 |
missense | 8 | 18 | 9 | 50 |
Any inclusion in genotype call
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 15 | 84 | 25 | 29 |
disruptive | 23 | 71 | 64 | 150 |
missense | 9 | 44 | 42 | 82 |
PlasmoDB Total SNPs: 176
Non-coding: 138 | Synonymous: 29 | Nonsynonymous: 9 | Stop Codon: 0
Protein Length: 718 | Molecular Weight (kDa): 81.484
UniProt ID(s): Q8I0V3
PDB ID(s): 7K3Z
Isoelectric Point: 4.71
Protein Domain Annotations:
Source | Family ID | Description |
---|---|---|
InterPro | IPR001844 | Chaperonin Cpn60 |
InterPro | IPR002423 | Chaperonin Cpn60/TCP-1 family |
InterPro | IPR027409 | GroEL-like apical domain superfamily |
InterPro | IPR027410 | TCP-1-like chaperonin intermediate domain superfamily |
InterPro | IPR027413 | GroEL-like equatorial domain superfamily |
PFam | PF00118 | Chaperonin Cpn60/TCP-1 family |
Superfamily | SSF48592 | GroEL-like equatorial domain superfamily |
Superfamily | SSF52029 | GroEL-like apical domain superfamily |
Superfamily | SSF54849 | TCP-1-like chaperonin intermediate domain superfamily |
PMID | Title | Authors | DOI/Link |
---|---|---|---|
12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
14747157 | The use of DsRED in single- and dual-color fluorescence labeling of mitochondrialand plastid organelles in Plasmodium falciparum. | Sato S, Wilson RJ | 10.1016/j.molbiopara.2003.11.015 |
16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
37350285 | Mycobacterial chaperonins in cellular proteostasis: Evidence for chaperonefunction of Cpn60.1 and Cpn60.2-mediated protein folding | Piplani B, Kumar CMS, Lund PA, Chaudhuri TK | 10.1111/mmi.15109 |