About Gene List

PF3D7_1232100 (CPN60)

Genome location: Pf3D7_12_v3:1,328,121..1,332,034(-)

Genome classification: Core

Function and Localization

Product Description: 60 kDa chaperonin

SignalP Peptide: MRMKRIHILLLVIFLLCLRYGYS

# Transmembrane Domains: 1

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020011 apicoplast
Component GO:0020020 food vacuole
Component GO:0005634 nucleus
Function GO:0003723 RNA binding
Function GO:0051082 unfolded protein binding
Process GO:0006457 protein folding

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring possibly expressed 0.27 0.88
Trophozoite expressed 1.40 0.35
Schizont expressed 0.81 0.55
Gametocyte expressed 0.74 0.61

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1232100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.148 | MFS: -2.575 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1446800)

  • Relative Growth Rate: 0.02 ± 0.18
  • Confidence: 4.82

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I0V3

"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=1.72) with 7K3Z (Global RMSD=3.41)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_130266

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 7 31 22 42
disruptive 16 31 16 68
missense 8 18 9 50

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 15 84 25 29
disruptive 23 71 64 150
missense 9 44 42 82

PlasmoDB Total SNPs: 176

Non-coding: 138 | Synonymous: 29 | Nonsynonymous: 9 | Stop Codon: 0

Protein Information

Protein Length: 718 | Molecular Weight (kDa): 81.484

UniProt ID(s): Q8I0V3

PDB ID(s): 7K3Z

Isoelectric Point: 4.71

Protein Domain Annotations:

Source Family ID Description
InterPro IPR001844 Chaperonin Cpn60
InterPro IPR002423 Chaperonin Cpn60/TCP-1 family
InterPro IPR027409 GroEL-like apical domain superfamily
InterPro IPR027410 TCP-1-like chaperonin intermediate domain superfamily
InterPro IPR027413 GroEL-like equatorial domain superfamily
PFam PF00118 Chaperonin Cpn60/TCP-1 family
Superfamily SSF48592 GroEL-like equatorial domain superfamily
Superfamily SSF52029 GroEL-like apical domain superfamily
Superfamily SSF54849 TCP-1-like chaperonin intermediate domain superfamily

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
14747157 The use of DsRED in single- and dual-color fluorescence labeling of mitochondrialand plastid organelles in Plasmodium falciparum. Sato S, Wilson RJ 10.1016/j.molbiopara.2003.11.015
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
21136929 Food vacuole proteome of the malarial parasite Plasmodium falciparum. Lamarque M, Tastet C, ..., Dubremetz JF 10.1002/prca.200700112
30212465 Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. Boucher MJ, Ghosh S, ..., Yeh E 10.1371/journal.pbio.2005895
30379851 Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. Briquet S, Ourimi A, ..., Vaquero C 10.1371/journal.pone.0205596
33906926 Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH Sinha A, Baumgarten S, ..., Scherf A 10.1128/mBio.00661-21
37350285 Mycobacterial chaperonins in cellular proteostasis: Evidence for chaperonefunction of Cpn60.1 and Cpn60.2-mediated protein folding Piplani B, Kumar CMS, Lund PA, Chaudhuri TK 10.1111/mmi.15109