PF3D7_1232400

Genome location: Pf3D7_12_v3:1,337,106..1,338,824(-)

Genome classification: Core

Function and Localization

Product Description: CWC16 domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005684	U2-type spliceosomal complex
Component	GO:0071014	post-mRNA release spliceosomal complex
Process	GO:0008380	RNA splicing

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	1.07	0.53
Trophozoite	expressed	0.57	0.68
Schizont	expressed	0.38	0.78
Gametocyte	expressed	0.85	0.56

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1232400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.758 | #Insertions: 2

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1447100)

Relative Growth Rate: 0.42 ± 0.14
Confidence: 5.35

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1447100)

Modification: Disrupted | RMgm-3984

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I598

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103127

Most Similar Human Ortholog: K7ELI4

TM-align score: 0.45 | RMSD: 3.49

Seq Identity: 0.23 | Length: 128 / 368

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000104957	coiled-coil domain containing 130

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	14	10	24
disruptive	19	42	28	70
missense	14	37	24	58

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	9	28	15	24
disruptive	40	111	56	138
missense	22	86	41	76

PlasmoDB Total SNPs: 62

Non-coding: 16 | Synonymous: 29 | Nonsynonymous: 16 | Stop Codon: 1

Protein Information

Protein Length: 368 | Molecular Weight (kDa): 44.07

UniProt ID(s): Q8I598

PDB ID(s): None

Isoelectric Point: 9.83

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007590	Saf4/Yju2 protein
PFam	PF04502	Saf4/Yju2 protein
Superfamily	N/A	N/A