PF3D7_1232900

Genome location: Pf3D7_12_v3:1,351,394..1,355,027(-)

Genome classification: Core

Function and Localization

Product Description: nucleotidyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.7.- (Nucleotidyltransferases.)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005634	nucleus
Function	GO:0016779	nucleotidyltransferase activity
Process	GO:0006397	mRNA processing

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.07	0.97
Trophozoite	expressed	0.24	0.86
Schizont	not expressed	0.05	0.97
Gametocyte	possibly expressed	0.23	0.86

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1232900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.124 | MFS: -3.025 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1447600)

Relative Growth Rate: 0.14 ± 0.11
Confidence: 5.84

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A144A2R0

"Best" AlphaFill ligand hit: 2KH (5'-o-[(s)-hydroxy{[(s)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]uridine, Local RMSD=0.71) with 5W0N (Global RMSD=5.26)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.7.-	Ex: 2-hydroxyethylphosphonate cytidylyltransferase	ADPAMPUTPKClNAD+NADP+NaAD+ppGpppppGppsuraminADPribosecisplatin...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101058

Most Similar Human Ortholog: Q5VYT0

TM-align score: 0.85 | RMSD: 2.08

Seq Identity: 0.22 | Length: 120 / 473

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000083223	terminal uridylyl transferase 7
ENSG00000134744	terminal uridylyl transferase 4

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	23	16	21
disruptive	5	24	21	39
missense	5	22	21	33

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	4	42	14	32
disruptive	9	55	34	84
missense	7	45	30	68

PlasmoDB Total SNPs: 149

Non-coding: 145 | Synonymous: 3 | Nonsynonymous: 1 | Stop Codon: 0

Protein Information

Protein Length: 473 | Molecular Weight (kDa): 55.862

UniProt ID(s): A0A144A2R0

PDB ID(s): None

Isoelectric Point: 9.58

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR043519	Nucleotidyltransferase superfamily
PFam	PF19088	N/A
Superfamily	SSF81301	Nucleotidyltransferase superfamily
Superfamily	SSF81631