Genome location: Pf3D7_12_v3:1,355,049..1,358,076(-)
Genome classification: Core
Product Description: elongation factor G
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Function | GO:0005525 | GTP binding |
| Function | GO:0003924 | GTPase activity |
| Function | GO:0003746 | translation elongation factor activity |
| Process | GO:0070125 | mitochondrial translational elongation |
| Process | GO:0006414 | translational elongation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.10 | 0.95 |
| Trophozoite | expressed | 0.51 | 0.70 |
| Schizont | not expressed | 0.17 | 0.89 |
| Gametocyte | not expressed | 0.42 | 0.76 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1233000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.119 | MFS: -3.012 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1447700)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I592
"Best" AlphaFill ligand hit: TAC (tetracycline, Local RMSD=0.23) with 2HDN (Global RMSD=5.16)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100770
Most Similar Human Ortholog: Q96RP9
TM-align score: 0.90 | RMSD: 2.47
Seq Identity: 0.43 | Length: 714 / 803
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000168827 | G elongation factor mitochondrial 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 39 | 10 | 45 |
| disruptive | 2 | 30 | 27 | 61 |
| missense | 2 | 30 | 27 | 53 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 72 | 23 | 42 |
| disruptive | 4 | 85 | 56 | 105 |
| missense | 3 | 76 | 48 | 80 |
PlasmoDB Total SNPs: 86
Non-coding: 62 | Synonymous: 16 | Nonsynonymous: 7 | Stop Codon: 1
Protein Length: 803 | Molecular Weight (kDa): 92.348
UniProt ID(s): C5J0C2, C5J0D6, Q8I592
PDB ID(s): None
Isoelectric Point: 8.54
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000640 | Elongation factor EFG, domain V-like |
| InterPro | IPR000795 | Transcription factor, GTP-binding domain |
| InterPro | IPR004161 | Translation elongation factor EFTu-like, domain 2 |
| InterPro | IPR004540 | Translation elongation factor EFG/EF2 |
| InterPro | IPR005225 | Small GTP-binding protein domain |
| InterPro | IPR005517 | Translation elongation factor EFG/EF2, domain IV |
| InterPro | IPR009000 | Translation protein, beta-barrel domain superfamily |
| InterPro | IPR009022 | Elongation factor G, domain III |
| InterPro | IPR020568 | Ribosomal protein S5 domain 2-type fold |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR031157 | Tr-type G domain, conserved site |
| InterPro | IPR035647 | EF-G domain III/V-like |
| InterPro | IPR035649 | EFG, domain V |
| InterPro | IPR041095 | Elongation Factor G, domain II |
| PFam | PF00009 | Transcription factor, GTP-binding domain |
| PFam | PF00679 | Elongation factor EFG, domain V-like |
| PFam | PF03144 | Translation elongation factor EFTu-like, domain 2 |
| PFam | PF03764 | Translation elongation factor EFG/EF2, domain IV |
| PFam | PF14492 | Elongation Factor G, domain II |
| Superfamily | SSF50447 | Translation protein, beta-barrel domain superfamily |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| Superfamily | SSF54211 | Ribosomal protein S5 domain 2-type fold |
| Superfamily | SSF54980 | EF-G domain III/V-like |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 21695207 | Characterization of two malaria parasite organelle translation elongation factorG proteins: the likely targets of the anti-malarial fusidic acid. | Johnson RA, McFadden GI, Goodman CD | 10.1371/journal.pone.0020633 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 38328191 | Multi-Channel smFRET study reveals a Compact conformation of EF-G on theRibosome | Johnson JL, Steele JH, ..., Wang Y | 10.1101/2024.01.27.577133 |
| 37159300 | Decoding and Recoding of mRNA Sequences by the Ribosome | Rodnina MV | 10.1146/annurev-biophys-101922-072452 |
| 37108045 | Interplay between Inter-Subunit Rotation of the Ribosome and Binding ofTranslational GTPases | Das A, Adiletta N, Ermolenko DN | 10.3390/ijms24086878 |
| 37214817 | Comparing FRET pairs that report on intersubunit rotation in bacterial ribosomes | Das A, Bao C, Ermolenko DN | 10.1101/2023.05.09.540051 |
| 36984696 | 3-Amino-Substituted Analogues of Fusidic Acid as Membrane-Active Antibacterial Compounds | Salimova EV, Mozgovoj OS, ..., Parfenova LV | 10.3390/membranes13030309 |
| 37648010 | Active role of the protein translation machinery in protecting against stress tolerance in Synechococcus elongatus PCC7942 | Ngoennet S, Sirisattha S, ..., Waditee-Sirisattha R | 10.1016/j.abb.2023.109734 |