Genome location: Pf3D7_12_v3:1,358,501..1,358,732(+)
Genome classification: Core
Product Description: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4, putative
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0008250 | oligosaccharyltransferase complex |
| Function | GO:0004579 | dolichyl-diphosphooligosaccharide-protein glycotransferase activity |
| Process | GO:0006487 | protein N-linked glycosylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | no data | N/A | N/A |
| Ring | no data | nd | nd |
| Trophozoite | no data | nd | nd |
| Schizont | no data | nd | nd |
| Gametocyte | no data | nd | nd |
More info:
No corresponding Pf3D7v3 gene ID in resistome database
Zhang Phenotype: N/A
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A5K1K906
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 2 | 1 | 2 |
| disruptive | 1 | 2 | 1 | 4 |
| missense | 1 | 2 | 1 | 4 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 2 | 2 | 1 |
| disruptive | 1 | 6 | 3 | 2 |
| missense | 1 | 4 | 2 | 2 |
PlasmoDB Total SNPs: 16
Non-coding: 15 | Synonymous: 1 | Nonsynonymous: 0 | Stop Codon: 0
Protein Length: 32 | Molecular Weight (kDa): 3.89
UniProt ID(s): A0A5K1K906
PDB ID(s): None
Isoelectric Point: 3.57
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR018943 | Oligosaccaryltransferase |
| InterPro | IPR036330 | Oligosaccharyltransferase subunit ost4p superfamily |
| PFam | PF10215 | Oligosaccaryltransferase |
| Superfamily | SSF103464 | Oligosaccharyltransferase subunit ost4p superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16096346 | The 3.4-kDa Ost4 protein is required for the assembly of two distinctoligosaccharyltransferase complexes in yeast | Spirig U, Bodmer D, ..., Aebi M | 10.1093/glycob/cwj025 |
| 23606741 | OST4 is a subunit of the mammalian oligosaccharyltransferase required forefficient N-glycosylation | Dumax-Vorzet A, Roboti P, High S | 10.1242/jcs.115410 |
| 21609714 | Solution structure of a human minimembrane protein Ost4, a subunit of theoligosaccharyltransferase complex | Gayen S, Kang C | 10.1016/j.bbrc.2011.05.050 |
| 16317064 | An evolving view of the eukaryotic oligosaccharyltransferase | Kelleher DJ, Gilmore R | 10.1093/glycob/cwj066 |
| 18955371 | The yeast oligosaccharyltransferase complex can be replaced by STT3 fromLeishmania major | Hese K, Otto C, Routier FH, Lehle L | 10.1093/glycob/cwn118 |
| 11870858 | Mutants defective in secretory/vacuolar pathways in the EUROFAN collection ofyeast disruptants | Avaro S, Belgareh-Touze N, ..., Haguenauer-Tsapis R | 10.1002/yea.838 |
| 33442744 | NMR and MD simulations reveal the impact of the V23D mutation on the function ofyeast oligosaccharyltransferase subunit Ost4 | Chaudhary BP, Zoetewey DL, McCullagh MJ, Mohanty S | 10.1093/glycob/cwab002 |
| 30835280 | An updated view of the oligosaccharyltransferase complex in Plasmodium | Tamana S, Promponas VJ | 10.1093/glycob/cwz011 |
| 12810948 | Determination of the membrane topology of Ost4p and its subunit interactions inthe oligosaccharyltransferase complex in Saccharomyces cerevisiae | Kim H, Yan Q, ..., Lennarz WJ | 10.1073/pnas.1332735100 |
| 38421550 | Reconstitution and resonance assignments of yeast OST subunit Ost4 and itscritical mutant Ost4V23D in liposomes by solid-state NMR | Chaudhary BP, Struppe J, ..., Mohanty S | 10.1007/s10858-024-00437-8 |
| 32328881 | (1)H, (13)C, (15)N resonance assignments and secondary structure of yeastoligosaccharyltransferase subunit Ost4 and its functionally important mutantOst4V23D | Chaudhary BP, Zoetewey D, Mohanty S | 10.1007/s12104-020-09946-7 |
| 14530272 | Insight into functional aspects of Stt3p, a subunit of the oligosaccharyltransferase. Evidence for interaction of the N-terminal domain of Stt3p with theprotein kinase C cascade | Chavan M, Rekowicz M, Lennarz W | 10.1074/jbc.M310456200 |
| 22116385 | A synthetic heparan sulfate oligosaccharide library reveals the novel enzymaticaction of D-glucosaminyl 3-O-sulfotransferase-3a | Nguyen TK, Arungundram S, ..., Kuberan B | 10.1039/c1mb05221g |